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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nitric oxide synthase oxygenase (nos) CSB-YP357832FKZ
CSB-EP357832FKZ
CSB-BP357832FKZ
CSB-MP357832FKZ
CSB-EP357832FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein ArsC (arsC) CSB-YP357833FKZ
CSB-EP357833FKZ
CSB-BP357833FKZ
CSB-MP357833FKZ
CSB-EP357833FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Potassium-transporting ATPase B chain 1 (kdpB1), partial CSB-YP357834SKY1
CSB-EP357834SKY1
CSB-BP357834SKY1
CSB-MP357834SKY1
CSB-EP357834SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tagatose 1,6-diphosphate aldolase (lacD) CSB-YP357835SKY
CSB-EP357835SKY
CSB-BP357835SKY
CSB-MP357835SKY
CSB-EP357835SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 10 kDa chaperonin (groS) CSB-YP357836SKX
CSB-EP357836SKX
CSB-BP357836SKX
CSB-MP357836SKX
CSB-EP357836SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP357837FKZ
CSB-EP357837FKZ
CSB-BP357837FKZ
CSB-MP357837FKZ
CSB-EP357837FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP357838SKX
CSB-EP357838SKX
CSB-BP357838SKX
CSB-MP357838SKX
CSB-EP357838SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP357839SUL
CSB-EP357839SUL
CSB-BP357839SUL
CSB-MP357839SUL
CSB-EP357839SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase III subunit beta (dnaN) CSB-YP357840SUL
CSB-EP357840SUL
CSB-BP357840SUL
CSB-MP357840SUL
CSB-EP357840SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cell division protein FtsZ (ftsZ) CSB-YP357841SKX
CSB-EP357841SKX
CSB-BP357841SKX
CSB-MP357841SKX
CSB-EP357841SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cell division protein FtsZ (ftsZ) CSB-YP357842FKZ
CSB-BP357842FKZ
CSB-MP357842FKZ
CSB-EP357842FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ferric uptake regulation protein (fur) CSB-YP357843SKY
CSB-EP357843SKY
CSB-BP357843SKY
CSB-MP357843SKY
CSB-EP357843SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glyceraldehyde-3-phosphate dehydrogenase 1 (gapA1) CSB-YP357844SKX
CSB-EP357844SKX
CSB-BP357844SKX
CSB-MP357844SKX
CSB-EP357844SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glyceraldehyde-3-phosphate dehydrogenase 1 (gapA1) CSB-YP357845FKZ
CSB-EP357845FKZ
CSB-BP357845FKZ
CSB-MP357845FKZ
CSB-EP357845FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillinase repressor (blaI) CSB-YP357846FKZ
CSB-EP357846FKZ
CSB-BP357846FKZ
CSB-MP357846FKZ
CSB-EP357846FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transposase for insertion sequence-like element IS431mec (tnp1) CSB-YP357847SKY
CSB-EP357847SKY
CSB-BP357847SKY
CSB-MP357847SKY
CSB-EP357847SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transposase for transposon Tn554 (tnpC1) CSB-YP357848SKX
CSB-EP357848SKX
CSB-BP357848SKX
CSB-MP357848SKX
CSB-EP357848SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for transposon Tn554 (tnpC) CSB-YP357849FKZ
CSB-EP357849FKZ
CSB-BP357849FKZ
CSB-MP357849FKZ
CSB-EP357849FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transposase A from transposon Tn554 (tnpA1) CSB-YP357850SKY
CSB-EP357850SKY
CSB-BP357850SKY
CSB-MP357850SKY
CSB-EP357850SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transposase B from transposon Tn554 (tnpB1), partial CSB-YP357851SKX
CSB-EP357851SKX
CSB-BP357851SKX
CSB-MP357851SKX
CSB-EP357851SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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