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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Neisseria meningitidis serogroup B Fimbrial protein (pilE) CSB-YP361519NGG
CSB-EP361519NGG
CSB-BP361519NGG
CSB-MP361519NGG
CSB-EP361519NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian sarcoma virus P47 (GAG-CRK) protein CSB-YP361520ATG
CSB-EP361520ATG
CSB-BP361520ATG
CSB-MP361520ATG
CSB-EP361520ATG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea ATP synthase gamma chain, chloroplastic (ATPC) CSB-YP361521FKI
CSB-EP361521FKI
CSB-BP361521FKI
CSB-MP361521FKI
CSB-EP361521FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain (atpC) CSB-YP361522RDU
CSB-EP361522RDU
CSB-BP361522RDU
CSB-MP361522RDU
CSB-EP361522RDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ClpA homolog protein, partial CSB-YP361523RDU
CSB-EP361523RDU
CSB-BP361523RDU
CSB-MP361523RDU
CSB-EP361523RDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative L,D-transpeptidase in ATP synthase subunits region ORF 5 CSB-YP361524RDU
CSB-EP361524RDU
CSB-BP361524RDU
CSB-MP361524RDU
CSB-EP361524RDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunits region ORF 7 CSB-YP361525RDU
CSB-EP361525RDU
CSB-BP361525RDU
CSB-MP361525RDU
CSB-EP361525RDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glutamine synthetase 1 (glnA) CSB-YP361526BVW
CSB-EP361526BVW
CSB-BP361526BVW
CSB-MP361526BVW
CSB-EP361526BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP361527ENV
CSB-EP361527ENV
CSB-BP361527ENV
CSB-MP361527ENV
CSB-EP361527ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P4 Glycoprotein 3 (sid) CSB-YP361528EDH
CSB-EP361528EDH
CSB-BP361528EDH
CSB-MP361528EDH
CSB-EP361528EDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P4 Derepression protein (Epsilon) CSB-YP361529EDH
CSB-EP361529EDH
CSB-BP361529EDH
CSB-MP361529EDH
CSB-EP361529EDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic (At2g45300) CSB-YP361530DOA
CSB-EP361530DOA
CSB-BP361530DOA
CSB-MP361530DOA
CSB-EP361530DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis DNA polymerase, partial CSB-YP361531KBK
CSB-EP361531KBK
CSB-BP361531KBK
CSB-MP361531KBK
CSB-EP361531KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Uncharacterized killer plasmid pGKL-2 helicase, partial CSB-YP361532KBK
CSB-EP361532KBK
CSB-BP361532KBK
CSB-MP361532KBK
CSB-EP361532KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Probable DNA-directed RNA polymerase, partial CSB-YP361533KBK
CSB-EP361533KBK
CSB-BP361533KBK
CSB-MP361533KBK
CSB-EP361533KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Uncharacterized killer plasmid pGKl-2 protein 8 CSB-YP361534KBK
CSB-EP361534KBK
CSB-BP361534KBK
CSB-MP361534KBK
CSB-EP361534KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis DNA-binding protein TRF1 (TRF1) CSB-YP361535KBK
CSB-EP361535KBK
CSB-BP361535KBK
CSB-MP361535KBK
CSB-EP361535KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max 18.5 kDa class I heat shock protein (HSP18.5-C) CSB-YP361536GGV
CSB-EP361536GGV
CSB-BP361536GGV
CSB-MP361536GGV
CSB-EP361536GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage phi80 Major head protein (E) CSB-YP361537EDL
CSB-EP361537EDL
CSB-BP361537EDL
CSB-MP361537EDL
CSB-EP361537EDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus geographus Mu-conotoxin GIIIC CSB-YP361538COM
CSB-EP361538COM
CSB-BP361538COM
CSB-MP361538COM
CSB-EP361538COM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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