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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Schizosaccharomyces pombe Plasma membrane ATPase 1 (pma1), partial CSB-YP362748SXV
CSB-EP362748SXV
CSB-BP362748SXV
CSB-MP362748SXV
CSB-EP362748SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myoxocephalus scorpius Peptide YY-like CSB-YP362749MMH
CSB-EP362749MMH
CSB-BP362749MMH
CSB-MP362749MMH
CSB-EP362749MMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Tubulin alpha-5 chain CSB-YP362750CH
CSB-EP362750CH
CSB-BP362750CH
CSB-MP362750CH
CSB-EP362750CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Guanyl-specific ribonuclease N1 (G15G9.140, NCU01045) CSB-YP362751NHA
CSB-EP362751NHA
CSB-BP362751NHA
CSB-MP362751NHA
CSB-EP362751NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Tubulin beta-5 chain CSB-YP362752CH
CSB-EP362752CH
CSB-BP362752CH
CSB-MP362752CH
CSB-EP362752CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Ribulose bisphosphate carboxylase small chain, chromosomal (cbxSC) CSB-YP362753CUS
CSB-EP362753CUS
CSB-BP362753CUS
CSB-MP362753CUS
CSB-EP362753CUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage SP Coat protein CSB-YP362755EDU
CSB-EP362755EDU
CSB-BP362755EDU
CSB-MP362755EDU
CSB-EP362755EDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage SP RNA-directed RNA polymerase beta chain, partial CSB-YP362756EDU
CSB-EP362756EDU
CSB-BP362756EDU
CSB-MP362756EDU
CSB-EP362756EDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage SP Readthrough protein A1 CSB-YP362757EDU
CSB-EP362757EDU
CSB-BP362757EDU
CSB-MP362757EDU
CSB-EP362757EDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein IRL4 CSB-YP362758HWV
CSB-EP362758HWV
CSB-BP362758HWV
CSB-MP362758HWV
CSB-EP362758HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein HHLF2 (US35) CSB-YP362759HWV
CSB-EP362759HWV
CSB-BP362759HWV
CSB-MP362759HWV
CSB-EP362759HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein HHLF4 CSB-YP362760HWV
CSB-EP362760HWV
CSB-BP362760HWV
CSB-MP362760HWV
CSB-EP362760HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Tegument protein US24 (US24), partial CSB-YP362761HWV
CSB-EP362761HWV
CSB-BP362761HWV
CSB-MP362761HWV
CSB-EP362761HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein HHRF1 CSB-YP362762HWV
CSB-EP362762HWV
CSB-BP362762HWV
CSB-MP362762HWV
CSB-EP362762HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein HHRF4 (US29) CSB-YP362763HWV
CSB-EP362763HWV
CSB-BP362763HWV
CSB-MP362763HWV
CSB-EP362763HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein HHRF6 (US31) CSB-YP362764HWV
CSB-EP362764HWV
CSB-BP362764HWV
CSB-MP362764HWV
CSB-EP362764HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Protein US34 (US34) CSB-YP362765HWV
CSB-EP362765HWV
CSB-BP362765HWV
CSB-MP362765HWV
CSB-EP362765HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein HKRFX CSB-YP362766HWV
CSB-EP362766HWV
CSB-BP362766HWV
CSB-MP362766HWV
CSB-EP362766HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Unique short US2 glycoprotein (US2), partial CSB-YP362767HWV
CSB-EP362767HWV
CSB-BP362767HWV
CSB-MP362767HWV
CSB-EP362767HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Protein IRS1 (IRS1), partial CSB-YP362768HWV
CSB-EP362768HWV
CSB-BP362768HWV
CSB-MP362768HWV
CSB-EP362768HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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