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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum Nitrogenase molybdenum-iron protein alpha chain (nifD) CSB-YP361721BVW
CSB-EP361721BVW
CSB-BP361721BVW
CSB-MP361721BVW
CSB-EP361721BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-like protein in vnf region CSB-YP361722DPU
CSB-EP361722DPU
CSB-BP361722DPU
CSB-MP361722DPU
CSB-EP361722DPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Vitamin B12 transporter BtuB (btuB), partial CSB-YP361723ENV
CSB-EP361723ENV
CSB-BP361723ENV
CSB-MP361723ENV
CSB-EP361723ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanobacterium formicicum Formate dehydrogenase subunit alpha (fdhA), partial CSB-YP361724MET
CSB-EP361724MET
CSB-BP361724MET
CSB-MP361724MET
CSB-EP361724MET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloramphenicol acetyltransferase (cat) CSB-YP361725FKZ
CSB-EP361725FKZ
CSB-BP361725FKZ
CSB-MP361725FKZ
CSB-EP361725FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit RLA class I histocompatibility antigen, alpha chain 19-1, partial CSB-YP361726RB
CSB-EP361726RB
CSB-BP361726RB
CSB-MP361726RB
CSB-EP361726RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Histone H2B.1, sperm CSB-YP361727FOT
CSB-EP361727FOT
CSB-BP361727FOT
CSB-MP361727FOT
CSB-EP361727FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage phi80 Probable regulatory protein N (N) CSB-YP361728EDL
CSB-EP361728EDL
CSB-BP361728EDL
CSB-MP361728EDL
CSB-EP361728EDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Newcastle disease virus Fusion glycoprotein F0 (F), partial CSB-YP361729NCO
CSB-EP361729NCO
CSB-BP361729NCO
CSB-MP361729NCO
CSB-EP361729NCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parainfluenza 3 virus Nucleoprotein (N), partial CSB-YP361730HPI
CSB-BP361730HPI
CSB-MP361730HPI
CSB-EP361730HPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human parainfluenza 3 virus Phosphoprotein (P/V/D), partial CSB-YP361731HPI
CSB-EP361731HPI
CSB-BP361731HPI
CSB-MP361731HPI
CSB-EP361731HPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine parainfluenza 3 virus Protein C (P/V/C) CSB-YP361732BKP
CSB-EP361732BKP
CSB-BP361732BKP
CSB-MP361732BKP
CSB-EP361732BKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine parainfluenza 3 virus Matrix protein (M) CSB-YP361733BKP
CSB-EP361733BKP
CSB-BP361733BKP
CSB-MP361733BKP
CSB-EP361733BKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ketol-acid reductoisomerase, mitochondrial (ILV5) CSB-YP361734SVG
CSB-EP361734SVG
CSB-BP361734SVG
CSB-MP361734SVG
CSB-EP361734SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transcriptional regulator Myc-A (myc-a) CSB-YP361735XBE
CSB-EP361735XBE
CSB-BP361735XBE
CSB-MP361735XBE
CSB-EP361735XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP361736SXB
CSB-EP361736SXB
CSB-BP361736SXB
CSB-MP361736SXB
CSB-EP361736SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phanerochaete chrysosporium Ligninase H8 (LPOA) CSB-YP361737EUK
CSB-EP361737EUK
CSB-BP361737EUK
CSB-MP361737EUK
CSB-EP361737EUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Photosystem II 10 kDa polypeptide, chloroplastic (PSBR) CSB-YP361738FIG
CSB-EP361738FIG
CSB-BP361738FIG
CSB-MP361738FIG
CSB-EP361738FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Outer membrane protease E (pgtE) CSB-YP361739SXB
CSB-EP361739SXB
CSB-BP361739SXB
CSB-MP361739SXB
CSB-EP361739SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Type-1 restriction enzyme StySJI specificity protein (hsdS) CSB-YP361740SXB
CSB-EP361740SXB
CSB-BP361740SXB
CSB-MP361740SXB
CSB-EP361740SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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