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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Barley yellow dwarf virus Putative RNA-directed RNA polymerase (ORF1/ORF2), partial CSB-YP362706BER
CSB-EP362706BER
CSB-BP362706BER
CSB-MP362706BER
CSB-EP362706BER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Turnip yellows virus Major capsid protein (ORF3) CSB-YP362707TJR
CSB-EP362707TJR
CSB-BP362707TJR
CSB-MP362707TJR
CSB-EP362707TJR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barley yellow dwarf virus Major capsid protein (ORF3) CSB-YP362708BER
CSB-EP362708BER
CSB-BP362708BER
CSB-MP362708BER
CSB-EP362708BER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet western yellows virus Movement protein (ORF4) CSB-YP362709BGC
CSB-EP362709BGC
CSB-BP362709BGC
CSB-MP362709BGC
CSB-EP362709BGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet western yellows virus Minor capsid protein P3-RTD (ORF3/ORF5), partial CSB-YP362710BGC
CSB-EP362710BGC
CSB-BP362710BGC
CSB-MP362710BGC
CSB-EP362710BGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barley yellow dwarf virus Minor capsid protein (ORF3/ORF5), partial CSB-YP362711BER
CSB-EP362711BER
CSB-BP362711BER
CSB-MP362711BER
CSB-EP362711BER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Figwort mosaic virus Probable capsid protein (ORF IV) CSB-YP362712FBJ
CSB-EP362712FBJ
CSB-BP362712FBJ
CSB-MP362712FBJ
CSB-EP362712FBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Figwort mosaic virus Aphid transmission protein (ORF II) CSB-YP362713FBJ
CSB-EP362713FBJ
CSB-BP362713FBJ
CSB-MP362713FBJ
CSB-EP362713FBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Figwort mosaic virus Enzymatic polyprotein (ORF V), partial CSB-YP362714FBJ
CSB-EP362714FBJ
CSB-BP362714FBJ
CSB-MP362714FBJ
CSB-EP362714FBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tripneustes gratilla Homeobox protein engrailed (EN) CSB-YP362715TIM
CSB-EP362715TIM
CSB-BP362715TIM
CSB-MP362715TIM
CSB-EP362715TIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein DVR-1 (dvr1) CSB-YP362716XBE
CSB-EP362716XBE
CSB-BP362716XBE
CSB-MP362716XBE
CSB-EP362716XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Hemolysin (hlyA), partial CSB-YP362718VEX
CSB-EP362718VEX
CSB-BP362718VEX
CSB-MP362718VEX
CSB-EP362718VEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae SWI/SNF chromatin-remodeling complex subunit SWI1 (ADR6), partial CSB-YP362719SVG
CSB-EP362719SVG
CSB-BP362719SVG
CSB-MP362719SVG
CSB-EP362719SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein dedD (dedD) CSB-YP362720ENV
CSB-EP362720ENV
CSB-BP362720ENV
CSB-MP362720ENV
CSB-EP362720ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lysine-arginine-ornithine-binding periplasmic protein (argT) CSB-YP362721ENV
CSB-EP362721ENV
CSB-BP362721ENV
CSB-MP362721ENV
CSB-EP362721ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein 2 (nifH2) CSB-YP362722CMA
CSB-EP362722CMA
CSB-BP362722CMA
CSB-MP362722CMA
CSB-EP362722CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein 3 (nifH3) CSB-YP362723CMA
CSB-EP362723CMA
CSB-BP362723CMA
CSB-MP362723CMA
CSB-EP362723CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein 5 (nifH5) CSB-YP362724CMA
CSB-EP362724CMA
CSB-BP362724CMA
CSB-MP362724CMA
CSB-EP362724CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein 6 (nifH6) CSB-YP362725CMA
CSB-EP362725CMA
CSB-BP362725CMA
CSB-MP362725CMA
CSB-EP362725CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Division inhibition protein dicB (dicB) CSB-YP362727ENV
CSB-EP362727ENV
CSB-BP362727ENV
CSB-MP362727ENV
CSB-EP362727ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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