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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Physarum polycephalum Spherulin-2A CSB-YP362665EVC
CSB-EP362665EVC
CSB-BP362665EVC
CSB-MP362665EVC
CSB-EP362665EVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus PS3 ATP synthase protein I (atpI), partial CSB-YP362666BBK1
CSB-EP362666BBK1
CSB-BP362666BBK1
CSB-MP362666BBK1
CSB-EP362666BBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type-2 restriction enzyme DpnII (dpnB) CSB-YP362667FMW
CSB-EP362667FMW
CSB-BP362667FMW
CSB-MP362667FMW
CSB-EP362667FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP362668EHU1
CSB-EP362668EHU1
CSB-BP362668EHU1
CSB-MP362668EHU1
CSB-EP362668EHU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sinapis alba Maturase K (matK), partial CSB-YP362669SZO
CSB-EP362669SZO
CSB-BP362669SZO
CSB-MP362669SZO
CSB-EP362669SZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 2 CSB-YP362670RGX
CSB-EP362670RGX
CSB-BP362670RGX
CSB-MP362670RGX
CSB-EP362670RGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Proline dehydrogenase, mitochondrial (PUT1), partial CSB-YP362671SVG
CSB-EP362671SVG
CSB-BP362671SVG
CSB-MP362671SVG
CSB-EP362671SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional activator rhaS (rhaS) CSB-YP362672ENV
CSB-EP362672ENV
CSB-BP362672ENV
CSB-MP362672ENV
CSB-EP362672ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional activator rhaR (rhaR) CSB-YP362673ENV
CSB-EP362673ENV
CSB-BP362673ENV
CSB-MP362673ENV
CSB-EP362673ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Single-stranded DNA-binding protein 1-B, mitochondrial (ssbp1-b) CSB-YP362674XBE
CSB-EP362674XBE
CSB-BP362674XBE
CSB-MP362674XBE
CSB-EP362674XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) CSB-YP362675ECC
CSB-EP362675ECC
CSB-BP362675ECC
CSB-MP362675ECC
CSB-EP362675ECC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 50S ribosomal protein L23, chloroplastic (rpl23-A) CSB-YP362676ZAX
CSB-EP362676ZAX
CSB-BP362676ZAX
CSB-MP362676ZAX
CSB-EP362676ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage SPbeta Modification methylase SPBetaI CSB-YP362677BQZ
CSB-EP362677BQZ
CSB-BP362677BQZ
CSB-MP362677BQZ
CSB-EP362677BQZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycerophosphoryl diester phosphodiesterase (glpQ) CSB-YP362678ENV
CSB-EP362678ENV
CSB-BP362678ENV
CSB-MP362678ENV
CSB-EP362678ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus X Movement and silencing protein TGBp1 (ORF2) CSB-YP362679PRC
CSB-EP362679PRC
CSB-BP362679PRC
CSB-MP362679PRC
CSB-EP362679PRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant dTDP-dihydrostreptose--streptidine-6-phosphate dihydrostreptosyltransferase (strH) CSB-YP362680SUW
CSB-EP362680SUW
CSB-BP362680SUW
CSB-MP362680SUW
CSB-EP362680SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Myosin, essential light chain (mlcE) CSB-YP362681DKK
CSB-EP362681DKK
CSB-BP362681DKK
CSB-MP362681DKK
CSB-EP362681DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis Phosphoglycerate kinase (pgk) CSB-YP362682ZBM
CSB-EP362682ZBM
CSB-BP362682ZBM
CSB-MP362682ZBM
CSB-EP362682ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Momordica charantia Trypsin inhibitor MCI-3 CSB-YP362683MHQ
CSB-EP362683MHQ
CSB-BP362683MHQ
CSB-MP362683MHQ
CSB-EP362683MHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Whey acidic protein (WAP) CSB-YP362684RB
CSB-EP362684RB
CSB-BP362684RB
CSB-MP362684RB
CSB-EP362684RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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