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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Peptide methionine sulfoxide reductase MsrA 2 (msrA2) CSB-YP362895FLF
CSB-EP362895FLF
CSB-BP362895FLF
CSB-MP362895FLF
CSB-EP362895FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP362896FLF
CSB-EP362896FLF
CSB-BP362896FLF
CSB-MP362896FLF
CSB-EP362896FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP362897SKY
CSB-EP362897SKY
CSB-BP362897SKY
CSB-MP362897SKY
CSB-EP362897SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nitric oxide synthase oxygenase (nos) CSB-YP362898SKX
CSB-EP362898SKX
CSB-BP362898SKX
CSB-MP362898SKX
CSB-EP362898SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nitric oxide synthase oxygenase (nos) CSB-YP362899SUL
CSB-EP362899SUL
CSB-BP362899SUL
CSB-MP362899SUL
CSB-EP362899SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcription antitermination protein NusG (nusG) CSB-YP362900SKY
CSB-EP362900SKY
CSB-BP362900SKY
CSB-MP362900SKY
CSB-EP362900SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aminoacyltransferase FemA (femA) CSB-YP362901SUL
CSB-EP362901SUL
CSB-BP362901SUL
CSB-MP362901SUL
CSB-EP362901SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Aminoacyltransferase FemB (femB) CSB-YP362902SKX
CSB-EP362902SKX
CSB-BP362902SKX
CSB-MP362902SKX
CSB-EP362902SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aminoacyltransferase FemB (femB) CSB-YP362903FKZ
CSB-EP362903FKZ
CSB-BP362903FKZ
CSB-MP362903FKZ
CSB-EP362903FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Methicillin resistance mecR1 protein (mecR1), partial CSB-YP362904SKY
CSB-EP362904SKY
CSB-BP362904SKY
CSB-MP362904SKY
CSB-EP362904SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methicillin resistance mecR1 protein (mecR1), partial CSB-YP362905FLL
CSB-EP362905FLL
CSB-BP362905FLL
CSB-MP362905FLL
CSB-EP362905FLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alkyl hydroperoxide reductase subunit C (ahpC) CSB-YP362906SKX
CSB-EP362906SKX
CSB-BP362906SKX
CSB-MP362906SKX
CSB-EP362906SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alkyl hydroperoxide reductase subunit C (ahpC) CSB-YP362907FLF
CSB-EP362907FLF
CSB-BP362907FLF
CSB-MP362907FLF
CSB-EP362907FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tagatose-6-phosphate kinase (lacC) CSB-YP362908FLF
CSB-EP362908FLF
CSB-BP362908FLF
CSB-MP362908FLF
CSB-EP362908FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional AAC/APH (aacA-aphD) CSB-YP362909FKZ
CSB-EP362909FKZ
CSB-BP362909FKZ
CSB-MP362909FKZ
CSB-EP362909FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein (repB) CSB-YP362910FKZ
CSB-EP362910FKZ
CSB-BP362910FKZ
CSB-MP362910FKZ
CSB-EP362910FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Plasmid recombination enzyme type 2 (pre) CSB-YP362911SKX
CSB-EP362911SKX
CSB-BP362911SKX
CSB-MP362911SKX
CSB-EP362911SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmid recombination enzyme type 2 (pre) CSB-YP362912FKZ
CSB-EP362912FKZ
CSB-BP362912FKZ
CSB-MP362912FKZ
CSB-EP362912FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication and maintenance protein (repL) CSB-YP362913FLT
CSB-EP362913FLT
CSB-BP362913FLT
CSB-MP362913FLT
CSB-EP362913FLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Streptomycin 3'-adenylyltransferase (ant1) CSB-YP362914SKY
CSB-EP362914SKY
CSB-BP362914SKY
CSB-MP362914SKY
CSB-EP362914SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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