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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Myoxocephalus scorpius Ice-structuring protein SS-3 CSB-YP366143MMH
CSB-EP366143MMH
CSB-BP366143MMH
CSB-MP366143MMH
CSB-EP366143MMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tree shrew adenovirus serotype 1 Hexon-associated protein (PIX) CSB-YP366144THL
CSB-EP366144THL
CSB-BP366144THL
CSB-MP366144THL
CSB-EP366144THL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulatory protein GAL4 (GAL4), partial CSB-YP366145SVG
CSB-EP366145SVG
CSB-BP366145SVG
CSB-MP366145SVG
CSB-EP366145SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 DNA adenine methylase (DAM) CSB-YP366146EDZ
CSB-EP366146EDZ
CSB-BP366146EDZ
CSB-MP366146EDZ
CSB-EP366146EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase EcoRV (ecoRVM) CSB-YP366147ENL
CSB-BP366147ENL
CSB-MP366147ENL
CSB-EP366147ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Protein synthesis inhibitor II (RIP30A) CSB-YP366148HWQ
CSB-EP366148HWQ
CSB-BP366148HWQ
CSB-MP366148HWQ
CSB-EP366148HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Serine/threonine-protein kinase US3 (US3) CSB-YP366149HWY
CSB-EP366149HWY
CSB-BP366149HWY
CSB-MP366149HWY
CSB-EP366149HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 DNA polymerase (43), partial CSB-YP366150EDZ
CSB-EP366150EDZ
CSB-BP366150EDZ
CSB-MP366150EDZ
CSB-EP366150EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius blomhoffii Phospholipase A2, basic CSB-YP366151GGN
CSB-EP366151GGN
CSB-BP366151GGN
CSB-MP366151GGN
CSB-EP366151GGN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig kappa chain V-I region BAN CSB-YP366152HU
CSB-EP366152HU
CSB-BP366152HU
CSB-MP366152HU
CSB-EP366152HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ig kappa chain V-I region Daudi CSB-YP366153HU
CSB-EP366153HU
CSB-BP366153HU
CSB-MP366153HU
CSB-EP366153HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-cell receptor alpha chain V region HPB-MLT CSB-YP366154HU
CSB-EP366154HU
CSB-BP366154HU
CSB-MP366154HU
CSB-EP366154HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-cell receptor alpha chain V region CTL-L17 (TCRA) CSB-YP366155HU
CSB-EP366155HU
CSB-BP366155HU
CSB-MP366155HU
CSB-EP366155HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Hemoglobin subunit beta-H1 (Hbb-bh1) CSB-YP366156MO
CSB-EP366156MO
CSB-BP366156MO
CSB-MP366156MO
CSB-EP366156MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L1 (rplA) CSB-YP366157GFM
CSB-EP366157GFM
CSB-BP366157GFM
CSB-MP366157GFM
CSB-EP366157GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L14 (rplN) CSB-YP366158GFM
CSB-EP366158GFM
CSB-BP366158GFM
CSB-MP366158GFM
CSB-EP366158GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L23 (rplW) CSB-YP366159GFM
CSB-EP366159GFM
CSB-BP366159GFM
CSB-MP366159GFM
CSB-EP366159GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 50S ribosomal protein L29 (rpmC) CSB-YP366160GFM
CSB-EP366160GFM
CSB-BP366160GFM
CSB-MP366160GFM
CSB-EP366160GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant VSG expression site-associated protein 117A CSB-YP366161TQV
CSB-EP366161TQV
CSB-BP366161TQV
CSB-MP366161TQV
CSB-EP366161TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter freundii Colicin-A (caa), partial CSB-YP366162DTO1
CSB-EP366162DTO1
CSB-BP366162DTO1
CSB-MP366162DTO1
CSB-EP366162DTO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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