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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria welshimeri serovar 6b Aspartate--tRNA ligase (aspS), partial CSB-YP366746LLT
CSB-EP366746LLT
CSB-BP366746LLT
CSB-MP366746LLT
CSB-EP366746LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP366747LLT
CSB-EP366747LLT
CSB-BP366747LLT
CSB-MP366747LLT
CSB-EP366747LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP366748LLT
CSB-EP366748LLT
CSB-BP366748LLT
CSB-MP366748LLT
CSB-EP366748LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b GTPase obg (obg) CSB-YP366749LLT
CSB-EP366749LLT
CSB-BP366749LLT
CSB-MP366749LLT
CSB-EP366749LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L27 (rpmA) CSB-YP366750LLT
CSB-EP366750LLT
CSB-BP366750LLT
CSB-MP366750LLT
CSB-EP366750LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L21 (rplU) CSB-YP366751LLT
CSB-EP366751LLT
CSB-BP366751LLT
CSB-MP366751LLT
CSB-EP366751LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Probable septum site-determining protein MinC (minC) CSB-YP366752LLT
CSB-EP366752LLT
CSB-BP366752LLT
CSB-MP366752LLT
CSB-EP366752LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP366753LLT
CSB-EP366753LLT
CSB-BP366753LLT
CSB-MP366753LLT
CSB-EP366753LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Probable GTP-binding protein EngB (engB) CSB-YP366754LLT
CSB-EP366754LLT
CSB-BP366754LLT
CSB-MP366754LLT
CSB-EP366754LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Transcriptional repressor NrdR (nrdR) CSB-YP366755LLT
CSB-EP366755LLT
CSB-BP366755LLT
CSB-MP366755LLT
CSB-EP366755LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP366756LLT
CSB-EP366756LLT
CSB-BP366756LLT
CSB-MP366756LLT
CSB-EP366756LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b UPF0173 metal-dependent hydrolase lwe1590 (lwe1590) CSB-YP366757LLT
CSB-EP366757LLT
CSB-BP366757LLT
CSB-MP366757LLT
CSB-EP366757LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Acetylglutamate kinase (argB) CSB-YP366758LLT
CSB-EP366758LLT
CSB-BP366758LLT
CSB-MP366758LLT
CSB-EP366758LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP366759LLT
CSB-EP366759LLT
CSB-BP366759LLT
CSB-MP366759LLT
CSB-EP366759LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Probable tRNA sulfurtransferase (thiI) CSB-YP366760LLT
CSB-EP366760LLT
CSB-BP366760LLT
CSB-MP366760LLT
CSB-EP366760LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S4 (rpsD) CSB-YP366761LLT
CSB-EP366761LLT
CSB-BP366761LLT
CSB-MP366761LLT
CSB-EP366761LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Tyrosine--tRNA ligase (tyrS) CSB-YP366762LLT
CSB-EP366762LLT
CSB-BP366762LLT
CSB-MP366762LLT
CSB-EP366762LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b UPF0354 protein lwe1624 (lwe1624) CSB-YP366763LLT
CSB-EP366763LLT
CSB-BP366763LLT
CSB-MP366763LLT
CSB-EP366763LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b tRNA (guanine-N (7)-)-methyltransferase CSB-YP366764LLT
CSB-EP366764LLT
CSB-BP366764LLT
CSB-MP366764LLT
CSB-EP366764LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Tryptophan synthase beta chain (trpB) CSB-YP366765LLT
CSB-EP366765LLT
CSB-BP366765LLT
CSB-MP366765LLT
CSB-EP366765LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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