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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L14 (rplN) CSB-YP366846LLT
CSB-EP366846LLT
CSB-BP366846LLT
CSB-MP366846LLT
CSB-EP366846LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L29 (rpmC) CSB-YP366847LLT
CSB-EP366847LLT
CSB-BP366847LLT
CSB-MP366847LLT
CSB-EP366847LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L16 (rplP) CSB-YP366848LLT
CSB-EP366848LLT
CSB-BP366848LLT
CSB-MP366848LLT
CSB-EP366848LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S19 (rpsS) CSB-YP366849LLT
CSB-EP366849LLT
CSB-BP366849LLT
CSB-MP366849LLT
CSB-EP366849LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L4 (rplD) CSB-YP366850LLT
CSB-EP366850LLT
CSB-BP366850LLT
CSB-MP366850LLT
CSB-EP366850LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S10 (rpsJ) CSB-YP366851LLT
CSB-EP366851LLT
CSB-BP366851LLT
CSB-MP366851LLT
CSB-EP366851LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S12 (rpsL) CSB-YP366852LLT
CSB-EP366852LLT
CSB-BP366852LLT
CSB-MP366852LLT
CSB-EP366852LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Potassium-transporting ATPase C chain (kdpC), partial CSB-YP366853LLT1
CSB-EP366853LLT1
CSB-BP366853LLT1
CSB-MP366853LLT1
CSB-EP366853LLT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Potassium-transporting ATPase A chain (kdpA), partial CSB-YP366854LLT
CSB-EP366854LLT
CSB-BP366854LLT
CSB-MP366854LLT
CSB-EP366854LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Serine--tRNA ligase (serS) CSB-YP366855LLT
CSB-EP366855LLT
CSB-BP366855LLT
CSB-MP366855LLT
CSB-EP366855LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Nucleoid occlusion protein (noc) CSB-YP366856LLT
CSB-EP366856LLT
CSB-BP366856LLT
CSB-MP366856LLT
CSB-EP366856LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP366857LLT
CSB-EP366857LLT
CSB-BP366857LLT
CSB-MP366857LLT
CSB-EP366857LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Ribonuclease P protein component (rnpA) CSB-YP366858LLT
CSB-EP366858LLT
CSB-BP366858LLT
CSB-MP366858LLT
CSB-EP366858LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Transmembrane emp24 domain-containing protein bai (bai), partial CSB-YP366859DMJ
CSB-EP366859DMJ
CSB-BP366859DMJ
CSB-MP366859DMJ
CSB-EP366859DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor TCP12 (TCP12) CSB-YP366860DOA
CSB-EP366860DOA
CSB-BP366860DOA
CSB-MP366860DOA
CSB-EP366860DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lens culinaris Non-specific lipid-transfer protein 2 CSB-YP366861LLM
CSB-EP366861LLM
CSB-BP366861LLM
CSB-MP366861LLM
CSB-EP366861LLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lens culinaris Non-specific lipid-transfer protein 5 CSB-YP366862LLM
CSB-EP366862LLM
CSB-BP366862LLM
CSB-MP366862LLM
CSB-EP366862LLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lens culinaris Non-specific lipid-transfer protein 6 CSB-YP366863LLM
CSB-EP366863LLM
CSB-BP366863LLM
CSB-MP366863LLM
CSB-EP366863LLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blackberry virus Y Genome polyprotein, partial CSB-YP366864BOY
CSB-EP366864BOY
CSB-BP366864BOY
CSB-MP366864BOY
CSB-EP366864BOY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. UPF0060 membrane protein Arth_4423 (Arth_4423), partial CSB-YP366865AVA1
CSB-EP366865AVA1
CSB-BP366865AVA1
CSB-MP366865AVA1
CSB-EP366865AVA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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