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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cenarchaeum symbiosum V-type ATP synthase subunit E (atpE) CSB-YP368266DRA
CSB-EP368266DRA
CSB-BP368266DRA
CSB-MP368266DRA
CSB-EP368266DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Probable exosome complex exonuclease 1 (CENSYa_1536) CSB-YP368267DRA
CSB-EP368267DRA
CSB-BP368267DRA
CSB-MP368267DRA
CSB-EP368267DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Proteasome subunit beta 2 (psmB2) CSB-YP368268DRA
CSB-EP368268DRA
CSB-BP368268DRA
CSB-MP368268DRA
CSB-EP368268DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum NAD (P)H-hydrate epimerase CSB-YP368269DRA
CSB-EP368269DRA
CSB-BP368269DRA
CSB-MP368269DRA
CSB-EP368269DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Orotate phosphoribosyltransferase (pyrE) CSB-YP368270DRA
CSB-EP368270DRA
CSB-BP368270DRA
CSB-MP368270DRA
CSB-EP368270DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum tRNA (cytidine (56)-2'-O)-methyltransferase CSB-YP368271DRA
CSB-EP368271DRA
CSB-BP368271DRA
CSB-MP368271DRA
CSB-EP368271DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum DNA-binding protein CENSYa_1764 (CENSYa_1764) CSB-YP368272DRA
CSB-EP368272DRA
CSB-BP368272DRA
CSB-MP368272DRA
CSB-EP368272DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Probable DNA primase small subunit (priA) CSB-YP368273DRA
CSB-EP368273DRA
CSB-BP368273DRA
CSB-MP368273DRA
CSB-EP368273DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cenarchaeum symbiosum Adenine deaminase (ade), partial CSB-YP368274DRA
CSB-EP368274DRA
CSB-BP368274DRA
CSB-MP368274DRA
CSB-EP368274DRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Transmembrane protein C7orf23 homolog, partial CSB-YP368275BO1
CSB-EP368275BO1
CSB-BP368275BO1
CSB-MP368275BO1
CSB-EP368275BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Regulated endocrine-specific protein 18 (RESP18) CSB-YP368276BO
CSB-EP368276BO
CSB-BP368276BO
CSB-MP368276BO
CSB-EP368276BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Redox-regulatory protein FAM213A (fam213a) CSB-YP368277XBF
CSB-EP368277XBF
CSB-BP368277XBF
CSB-MP368277XBF
CSB-EP368277XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uncharacterized protein C14orf166B homolog CSB-YP368278RA
CSB-EP368278RA
CSB-BP368278RA
CSB-MP368278RA
CSB-EP368278RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AP2-like ethylene-responsive transcription factor At1g79700 (At1g79700) CSB-YP368279DOA
CSB-EP368279DOA
CSB-BP368279DOA
CSB-MP368279DOA
CSB-EP368279DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. UPF0271 protein Arth_0093 (Arth_0093) CSB-YP368280AVA
CSB-EP368280AVA
CSB-BP368280AVA
CSB-MP368280AVA
CSB-EP368280AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Pantothenate synthetase (panC) CSB-YP368281AVA
CSB-EP368281AVA
CSB-BP368281AVA
CSB-MP368281AVA
CSB-EP368281AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Urease accessory protein UreF (ureF) CSB-YP368282AVA
CSB-EP368282AVA
CSB-BP368282AVA
CSB-MP368282AVA
CSB-EP368282AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Uracil phosphoribosyltransferase (upp) CSB-YP368283AVA
CSB-EP368283AVA
CSB-BP368283AVA
CSB-MP368283AVA
CSB-EP368283AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Cysteine--tRNA ligase (cysS) CSB-YP368284AVA
CSB-EP368284AVA
CSB-BP368284AVA
CSB-MP368284AVA
CSB-EP368284AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Biotin synthase (bioB) CSB-YP368285AVA
CSB-EP368285AVA
CSB-BP368285AVA
CSB-MP368285AVA
CSB-EP368285AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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