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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arthrobacter sp. UPF0317 protein Arth_3576 (Arth_3576) CSB-YP368886AVA
CSB-EP368886AVA
CSB-BP368886AVA
CSB-MP368886AVA
CSB-EP368886AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Carboxylate-amine ligase Arth_3862 (Arth_3862) CSB-YP368887AVA
CSB-EP368887AVA
CSB-BP368887AVA
CSB-MP368887AVA
CSB-EP368887AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L33 (rpmG) CSB-YP368888AVA
CSB-EP368888AVA
CSB-BP368888AVA
CSB-MP368888AVA
CSB-EP368888AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L9 (rplI) CSB-YP368889AVA
CSB-EP368889AVA
CSB-BP368889AVA
CSB-MP368889AVA
CSB-EP368889AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L34 (rpmH) CSB-YP368890AVA
CSB-EP368890AVA
CSB-BP368890AVA
CSB-MP368890AVA
CSB-EP368890AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Putative pterin-4-alpha-carbinolamine dehydratase (Bcen2424_0077) CSB-YP368891BPO
CSB-EP368891BPO
CSB-BP368891BPO
CSB-MP368891BPO
CSB-EP368891BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 50S ribosomal protein L1 (rplA) CSB-YP368892BPO
CSB-EP368892BPO
CSB-BP368892BPO
CSB-MP368892BPO
CSB-EP368892BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP368893BPO
CSB-EP368893BPO
CSB-BP368893BPO
CSB-MP368893BPO
CSB-EP368893BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S10 (rpsJ) CSB-YP368894BPO
CSB-EP368894BPO
CSB-BP368894BPO
CSB-MP368894BPO
CSB-EP368894BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S19 (rpsS) CSB-YP368895BPO
CSB-EP368895BPO
CSB-BP368895BPO
CSB-MP368895BPO
CSB-EP368895BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S17 (rpsQ) CSB-YP368896BPO
CSB-EP368896BPO
CSB-BP368896BPO
CSB-MP368896BPO
CSB-EP368896BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S8 (rpsH) CSB-YP368897BPO
CSB-EP368897BPO
CSB-BP368897BPO
CSB-MP368897BPO
CSB-EP368897BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 50S ribosomal protein L30 (rpmD) CSB-YP368898BPO
CSB-EP368898BPO
CSB-BP368898BPO
CSB-MP368898BPO
CSB-EP368898BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 50S ribosomal protein L36 (rpmJ) CSB-YP368899BPO
CSB-EP368899BPO
CSB-BP368899BPO
CSB-MP368899BPO
CSB-EP368899BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S11 (rpsK) CSB-YP368900BPO
CSB-EP368900BPO
CSB-BP368900BPO
CSB-MP368900BPO
CSB-EP368900BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Probable GTP-binding protein EngB (engB) CSB-YP368901BPO
CSB-EP368901BPO
CSB-BP368901BPO
CSB-MP368901BPO
CSB-EP368901BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia sn-glycerol-3-phosphate import ATP-binding protein UgpC (ugpC) CSB-YP368902BPO
CSB-EP368902BPO
CSB-BP368902BPO
CSB-MP368902BPO
CSB-EP368902BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia ATP phosphoribosyltransferase (hisG) CSB-YP368903BPO
CSB-EP368903BPO
CSB-BP368903BPO
CSB-MP368903BPO
CSB-EP368903BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP368904BPO
CSB-EP368904BPO
CSB-BP368904BPO
CSB-MP368904BPO
CSB-EP368904BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Indole-3-glycerol phosphate synthase (trpC) CSB-YP368905BPO
CSB-EP368905BPO
CSB-BP368905BPO
CSB-MP368905BPO
CSB-EP368905BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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