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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 2-keto-3-deoxy-L-rhamnonate aldolase (rhmA) CSB-YP372288EJE
CSB-EP372288EJE
CSB-BP372288EJE
CSB-MP372288EJE
CSB-EP372288EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-rhamnonate dehydratase (rhmD) CSB-YP372289EJE
CSB-EP372289EJE
CSB-BP372289EJE
CSB-MP372289EJE
CSB-EP372289EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP372290EJE
CSB-EP372290EJE
CSB-BP372290EJE
CSB-MP372290EJE
CSB-EP372290EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional polymyxin resistance protein ArnA (arnA) CSB-YP372291EJE
CSB-EP372291EJE
CSB-BP372291EJE
CSB-MP372291EJE
CSB-EP372291EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP372292EJE
CSB-EP372292EJE
CSB-BP372292EJE
CSB-MP372292EJE
CSB-EP372292EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP372293EJE1
CSB-EP372293EJE1
CSB-BP372293EJE1
CSB-MP372293EJE1
CSB-EP372293EJE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP372294EJE
CSB-EP372294EJE
CSB-BP372294EJE
CSB-MP372294EJE
CSB-EP372294EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease BN (rbn) CSB-YP372295EJE
CSB-EP372295EJE
CSB-BP372295EJE
CSB-MP372295EJE
CSB-EP372295EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP372296EJE
CSB-EP372296EJE
CSB-BP372296EJE
CSB-MP372296EJE
CSB-EP372296EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit C/D (nuoC) CSB-YP372297EJE
CSB-EP372297EJE
CSB-BP372297EJE
CSB-MP372297EJE
CSB-EP372297EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0207 protein yfbR (yfbR) CSB-YP372298EJE
CSB-EP372298EJE
CSB-BP372298EJE
CSB-MP372298EJE
CSB-EP372298EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) CSB-YP372299EJE
CSB-EP372299EJE
CSB-BP372299EJE
CSB-MP372299EJE
CSB-EP372299EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fatty acid oxidation complex subunit alpha (fadJ) CSB-YP372300EJE
CSB-EP372300EJE
CSB-BP372300EJE
CSB-MP372300EJE
CSB-EP372300EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-serine dehydratase (dsdA) CSB-YP372301EJE
CSB-EP372301EJE
CSB-BP372301EJE
CSB-MP372301EJE
CSB-EP372301EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ion-transport protein yfeO (yfeO), partial CSB-YP372302EJE
CSB-EP372302EJE
CSB-BP372302EJE
CSB-MP372302EJE
CSB-EP372302EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate--tRNA ligase (gltX) CSB-YP372303EJE
CSB-EP372303EJE
CSB-BP372303EJE
CSB-MP372303EJE
CSB-EP372303EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein ZipA homolog (zipA), partial CSB-YP372304EJE
CSB-EP372304EJE
CSB-BP372304EJE
CSB-MP372304EJE
CSB-EP372304EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxine kinase (pdxK) CSB-YP372305EJE
CSB-EP372305EJE
CSB-BP372305EJE
CSB-MP372305EJE
CSB-EP372305EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP372306EJE
CSB-EP372306EJE
CSB-BP372306EJE
CSB-MP372306EJE
CSB-EP372306EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GDP-mannose pyrophosphatase nudK (nudK) CSB-YP372307EJE
CSB-EP372307EJE
CSB-BP372307EJE
CSB-MP372307EJE
CSB-EP372307EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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