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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorobium phaeobacteroides Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP372688CWO
CSB-EP372688CWO
CSB-BP372688CWO
CSB-MP372688CWO
CSB-EP372688CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP372689CWO
CSB-EP372689CWO
CSB-BP372689CWO
CSB-MP372689CWO
CSB-EP372689CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP372690CWO
CSB-EP372690CWO
CSB-BP372690CWO
CSB-MP372690CWO
CSB-EP372690CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides CinA-like protein (Cpha266_2541) CSB-YP372691CWO
CSB-EP372691CWO
CSB-BP372691CWO
CSB-MP372691CWO
CSB-EP372691CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP372692CWO
CSB-EP372692CWO
CSB-BP372692CWO
CSB-MP372692CWO
CSB-EP372692CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides DNA repair protein recO (recO) CSB-YP372693CWO
CSB-EP372693CWO
CSB-BP372693CWO
CSB-MP372693CWO
CSB-EP372693CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP372694CWO
CSB-EP372694CWO
CSB-BP372694CWO
CSB-MP372694CWO
CSB-EP372694CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP372695CWO
CSB-EP372695CWO
CSB-BP372695CWO
CSB-MP372695CWO
CSB-EP372695CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP372696CWO
CSB-EP372696CWO
CSB-BP372696CWO
CSB-MP372696CWO
CSB-EP372696CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP372697CWO
CSB-EP372697CWO
CSB-BP372697CWO
CSB-MP372697CWO
CSB-EP372697CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE), partial CSB-YP372698CWO
CSB-EP372698CWO
CSB-BP372698CWO
CSB-MP372698CWO
CSB-EP372698CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Ribonuclease P protein component (rnpA) CSB-YP372699CWO
CSB-EP372699CWO
CSB-BP372699CWO
CSB-MP372699CWO
CSB-EP372699CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus marmoreus Cysteine-rich secretory protein Mr30 CSB-YP372700DWD
CSB-EP372700DWD
CSB-BP372700DWD
CSB-MP372700DWD
CSB-EP372700DWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Accessory Sec system protein translocase subunit SecY2 (secY2), partial CSB-YP372701FMV
CSB-EP372701FMV
CSB-BP372701FMV
CSB-MP372701FMV
CSB-EP372701FMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Protein sip5 (sip5), partial CSB-YP372702AVC
CSB-EP372702AVC
CSB-BP372702AVC
CSB-MP372702AVC
CSB-EP372702AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable pectate lyase E (plyE) CSB-YP372703AVC
CSB-EP372703AVC
CSB-BP372703AVC
CSB-MP372703AVC
CSB-EP372703AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus ATP-dependent RNA helicase dbp3 (dbp3), partial CSB-YP372704AVC
CSB-EP372704AVC
CSB-BP372704AVC
CSB-MP372704AVC
CSB-EP372704AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Riboflavin kinase (fmn1) CSB-YP372705AVC
CSB-EP372705AVC
CSB-BP372705AVC
CSB-MP372705AVC
CSB-EP372705AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mannitol-1-phosphate 5-dehydrogenase (mpdA) CSB-YP372706AVC
CSB-EP372706AVC
CSB-BP372706AVC
CSB-MP372706AVC
CSB-EP372706AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Amino-acid acetyltransferase, mitochondrial (arg2), partial CSB-YP372707AVC
CSB-EP372707AVC
CSB-BP372707AVC
CSB-MP372707AVC
CSB-EP372707AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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