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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pelobacter propionicus Bifunctional protein GlmU (glmU) CSB-YP373148PYF
CSB-EP373148PYF
CSB-BP373148PYF
CSB-MP373148PYF
CSB-EP373148PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus UPF0313 protein Ppro_0556 (Ppro_0556), partial CSB-YP373149PYF
CSB-EP373149PYF
CSB-BP373149PYF
CSB-MP373149PYF
CSB-EP373149PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus UPF0210 protein Ppro_0613 (Ppro_0613) CSB-YP373150PYF
CSB-EP373150PYF
CSB-BP373150PYF
CSB-MP373150PYF
CSB-EP373150PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP373151PYF
CSB-EP373151PYF
CSB-BP373151PYF
CSB-MP373151PYF
CSB-EP373151PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L1 (rplA) CSB-YP373152PYF
CSB-EP373152PYF
CSB-BP373152PYF
CSB-MP373152PYF
CSB-EP373152PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP373153PYF
CSB-EP373153PYF
CSB-BP373153PYF
CSB-MP373153PYF
CSB-EP373153PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L3 (rplC) CSB-YP373154PYF
CSB-EP373154PYF
CSB-BP373154PYF
CSB-MP373154PYF
CSB-EP373154PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L23 (rplW) CSB-YP373155PYF
CSB-EP373155PYF
CSB-BP373155PYF
CSB-MP373155PYF
CSB-EP373155PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 30S ribosomal protein S3 (rpsC) CSB-YP373156PYF
CSB-EP373156PYF
CSB-BP373156PYF
CSB-MP373156PYF
CSB-EP373156PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L24 (rplX) CSB-YP373157PYF
CSB-EP373157PYF
CSB-BP373157PYF
CSB-MP373157PYF
CSB-EP373157PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L6 (rplF) CSB-YP373158PYF
CSB-EP373158PYF
CSB-BP373158PYF
CSB-MP373158PYF
CSB-EP373158PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L15 (rplO) CSB-YP373159PYF
CSB-EP373159PYF
CSB-BP373159PYF
CSB-MP373159PYF
CSB-EP373159PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 30S ribosomal protein S4 (rpsD) CSB-YP373160PYF
CSB-EP373160PYF
CSB-BP373160PYF
CSB-MP373160PYF
CSB-EP373160PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP373161PYF
CSB-EP373161PYF
CSB-BP373161PYF
CSB-MP373161PYF
CSB-EP373161PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 30S ribosomal protein S6 (rpsF) CSB-YP373162PYF
CSB-EP373162PYF
CSB-BP373162PYF
CSB-MP373162PYF
CSB-EP373162PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus ATP synthase subunit a 2 (atpB2), partial CSB-YP373163PYF1
CSB-EP373163PYF1
CSB-BP373163PYF1
CSB-MP373163PYF1
CSB-EP373163PYF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Ribosome maturation factor RimP (rimP) CSB-YP373164PYF
CSB-EP373164PYF
CSB-BP373164PYF
CSB-MP373164PYF
CSB-EP373164PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP373165PYF
CSB-EP373165PYF
CSB-BP373165PYF
CSB-MP373165PYF
CSB-EP373165PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus DNA mismatch repair protein MutS (mutS), partial CSB-YP373166PYF
CSB-EP373166PYF
CSB-BP373166PYF
CSB-MP373166PYF
CSB-EP373166PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 30S ribosomal protein S16 (rpsP) CSB-YP373167PYF
CSB-EP373167PYF
CSB-BP373167PYF
CSB-MP373167PYF
CSB-EP373167PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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