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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 10 kDa chaperonin (groS) CSB-YP375089YAK
CSB-EP375089YAK
CSB-BP375089YAK
CSB-MP375089YAK
CSB-EP375089YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Elongation factor P (efp) CSB-YP375090YAK
CSB-EP375090YAK
CSB-BP375090YAK
CSB-MP375090YAK
CSB-EP375090YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B DNA mismatch repair protein mutL (mutL), partial CSB-YP375091YAK
CSB-EP375091YAK
CSB-BP375091YAK
CSB-MP375091YAK
CSB-EP375091YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B HTH-type transcriptional repressor NsrR (nsrR) CSB-YP375092YAK
CSB-EP375092YAK
CSB-BP375092YAK
CSB-MP375092YAK
CSB-EP375092YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 50S ribosomal protein L9 (rplI) CSB-YP375093YAK
CSB-EP375093YAK
CSB-BP375093YAK
CSB-MP375093YAK
CSB-EP375093YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP375094YAK
CSB-EP375094YAK
CSB-BP375094YAK
CSB-MP375094YAK
CSB-EP375094YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 50S ribosomal protein L27 (rpmA) CSB-YP375095YAK
CSB-EP375095YAK
CSB-BP375095YAK
CSB-MP375095YAK
CSB-EP375095YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP375096YAK
CSB-EP375096YAK
CSB-BP375096YAK
CSB-MP375096YAK
CSB-EP375096YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP375097YAK
CSB-EP375097YAK
CSB-BP375097YAK
CSB-MP375097YAK
CSB-EP375097YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Uncharacterized aldolase lsrF (lsrF) CSB-YP375098YAK
CSB-EP375098YAK
CSB-BP375098YAK
CSB-MP375098YAK
CSB-EP375098YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Autoinducer 2 kinase LsrK (lsrK), partial CSB-YP375099YAK
CSB-EP375099YAK
CSB-BP375099YAK
CSB-MP375099YAK
CSB-EP375099YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Peptide chain release factor 3 (prfC), partial CSB-YP375100YAK
CSB-EP375100YAK
CSB-BP375100YAK
CSB-MP375100YAK
CSB-EP375100YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Trp operon repressor (trpR) CSB-YP375101YAK
CSB-EP375101YAK
CSB-BP375101YAK
CSB-MP375101YAK
CSB-EP375101YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP375102YAK
CSB-EP375102YAK
CSB-BP375102YAK
CSB-MP375102YAK
CSB-EP375102YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 30S ribosomal protein S20 (rpsT) CSB-YP375103YAK
CSB-EP375103YAK
CSB-BP375103YAK
CSB-MP375103YAK
CSB-EP375103YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Dihydrodipicolinate reductase (dapB) CSB-YP375104YAK
CSB-EP375104YAK
CSB-BP375104YAK
CSB-MP375104YAK
CSB-EP375104YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP375105YAK
CSB-EP375105YAK
CSB-BP375105YAK
CSB-MP375105YAK
CSB-EP375105YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP375106YAK
CSB-EP375106YAK
CSB-BP375106YAK
CSB-MP375106YAK
CSB-EP375106YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B HTH-type transcriptional regulator sgrR (sgrR), partial CSB-YP375107YAK
CSB-EP375107YAK
CSB-BP375107YAK
CSB-MP375107YAK
CSB-EP375107YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Protein MraZ (mraZ) CSB-YP375108YAK
CSB-EP375108YAK
CSB-BP375108YAK
CSB-MP375108YAK
CSB-EP375108YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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