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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Glycogen synthase (glgA) CSB-YP374328YAK
CSB-EP374328YAK
CSB-BP374328YAK
CSB-MP374328YAK
CSB-EP374328YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP374329YAK
CSB-EP374329YAK
CSB-BP374329YAK
CSB-MP374329YAK
CSB-EP374329YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0194 membrane protein YE2891 (YE2891) CSB-YP374330YAK
CSB-EP374330YAK
CSB-BP374330YAK
CSB-MP374330YAK
CSB-EP374330YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Flagellar protein FliT (fliT) CSB-YP374331YAK
CSB-EP374331YAK
CSB-BP374331YAK
CSB-MP374331YAK
CSB-EP374331YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Flagellar hook-basal body complex protein FliE (fliE) CSB-YP374332YAK
CSB-EP374332YAK
CSB-BP374332YAK
CSB-MP374332YAK
CSB-EP374332YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Xylose isomerase (xylA) CSB-YP374333YAK
CSB-EP374333YAK
CSB-BP374333YAK
CSB-MP374333YAK
CSB-EP374333YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Flagella synthesis protein flgN (flgN) CSB-YP374334YAK
CSB-EP374334YAK
CSB-BP374334YAK
CSB-MP374334YAK
CSB-EP374334YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP374335YAK
CSB-EP374335YAK
CSB-BP374335YAK
CSB-MP374335YAK
CSB-EP374335YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Chromosomal replication initiator protein DnaA (dnaA) CSB-YP374336YAK
CSB-EP374336YAK
CSB-BP374336YAK
CSB-MP374336YAK
CSB-EP374336YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B ATP synthase subunit c (atpE), partial CSB-YP374337YAK1
CSB-EP374337YAK1
CSB-BP374337YAK1
CSB-MP374337YAK1
CSB-EP374337YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Pesticin receptor (fyuA), partial CSB-YP374338YAK
CSB-EP374338YAK
CSB-BP374338YAK
CSB-MP374338YAK
CSB-EP374338YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP374339YAK
CSB-EP374339YAK
CSB-BP374339YAK
CSB-MP374339YAK
CSB-EP374339YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Probable propionate kinase (pduW) CSB-YP374340YAK
CSB-EP374340YAK
CSB-BP374340YAK
CSB-MP374340YAK
CSB-EP374340YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0265 protein YE2755 (YE2755) CSB-YP374341YAK
CSB-EP374341YAK
CSB-BP374341YAK
CSB-MP374341YAK
CSB-EP374341YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Histidinol-phosphate aminotransferase (hisC) CSB-YP374342YAK
CSB-EP374342YAK
CSB-BP374342YAK
CSB-MP374342YAK
CSB-EP374342YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Uridine kinase (udk) CSB-YP374343YAK
CSB-EP374343YAK
CSB-BP374343YAK
CSB-MP374343YAK
CSB-EP374343YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP374344YAK
CSB-EP374344YAK
CSB-BP374344YAK
CSB-MP374344YAK
CSB-EP374344YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Chaperone protein sycT (sycT) CSB-YP374345YAK
CSB-EP374345YAK
CSB-BP374345YAK
CSB-MP374345YAK
CSB-EP374345YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Chaperone protein yscY (yscY) CSB-YP374346YAK
CSB-EP374346YAK
CSB-BP374346YAK
CSB-MP374346YAK
CSB-EP374346YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP374347AVL
CSB-EP374347AVL
CSB-BP374347AVL
CSB-MP374347AVL
CSB-EP374347AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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