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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Psychromonas ingrahamii ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP376589PZS
CSB-EP376589PZS
CSB-BP376589PZS
CSB-MP376589PZS
CSB-EP376589PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Elongation factor P-like protein (Ping_1470) CSB-YP376590PZS
CSB-EP376590PZS
CSB-BP376590PZS
CSB-MP376590PZS
CSB-EP376590PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP376591PZS
CSB-EP376591PZS
CSB-BP376591PZS
CSB-MP376591PZS
CSB-EP376591PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Glutamyl-tRNA reductase (hemA) CSB-YP376592PZS
CSB-EP376592PZS
CSB-BP376592PZS
CSB-MP376592PZS
CSB-EP376592PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP376593PZS
CSB-EP376593PZS
CSB-BP376593PZS
CSB-MP376593PZS
CSB-EP376593PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Methionine--tRNA ligase (metG), partial CSB-YP376594PZS
CSB-EP376594PZS
CSB-BP376594PZS
CSB-MP376594PZS
CSB-EP376594PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP376595PZS
CSB-EP376595PZS
CSB-BP376595PZS
CSB-MP376595PZS
CSB-EP376595PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii ATP phosphoribosyltransferase (hisG) CSB-YP376596PZS
CSB-EP376596PZS
CSB-BP376596PZS
CSB-MP376596PZS
CSB-EP376596PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP376597PZS
CSB-EP376597PZS
CSB-BP376597PZS
CSB-MP376597PZS
CSB-EP376597PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Dihydrodipicolinate synthase (dapA) CSB-YP376598PZS
CSB-EP376598PZS
CSB-BP376598PZS
CSB-MP376598PZS
CSB-EP376598PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Biotin synthase (bioB) CSB-YP376599PZS
CSB-EP376599PZS
CSB-BP376599PZS
CSB-MP376599PZS
CSB-EP376599PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Beta-hexosaminidase (nagZ) CSB-YP376600PZS
CSB-EP376600PZS
CSB-BP376600PZS
CSB-MP376600PZS
CSB-EP376600PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Probable transcriptional regulatory protein Ping_2063 (Ping_2063) CSB-YP376601PZS
CSB-EP376601PZS
CSB-BP376601PZS
CSB-MP376601PZS
CSB-EP376601PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP376602PZS
CSB-EP376602PZS
CSB-BP376602PZS
CSB-MP376602PZS
CSB-EP376602PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP376603PZS
CSB-EP376603PZS
CSB-BP376603PZS
CSB-MP376603PZS
CSB-EP376603PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Protein proQ homolog (proQ) CSB-YP376604PZS
CSB-EP376604PZS
CSB-BP376604PZS
CSB-MP376604PZS
CSB-EP376604PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP376605PZS
CSB-EP376605PZS
CSB-BP376605PZS
CSB-MP376605PZS
CSB-EP376605PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP376606PZS
CSB-EP376606PZS
CSB-BP376606PZS
CSB-MP376606PZS
CSB-EP376606PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP376607PZS
CSB-EP376607PZS
CSB-BP376607PZS
CSB-MP376607PZS
CSB-EP376607PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Recombination protein RecR (recR) CSB-YP376608PZS
CSB-EP376608PZS
CSB-BP376608PZS
CSB-MP376608PZS
CSB-EP376608PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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