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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Azoarcus sp. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP374829AVL
CSB-EP374829AVL
CSB-BP374829AVL
CSB-MP374829AVL
CSB-EP374829AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. LPS-assembly protein lptD (lptD), partial CSB-YP374830AVL
CSB-EP374830AVL
CSB-BP374830AVL
CSB-MP374830AVL
CSB-EP374830AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Ribosome maturation factor RimM (rimM) CSB-YP374831AVL
CSB-EP374831AVL
CSB-BP374831AVL
CSB-MP374831AVL
CSB-EP374831AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Catalase-peroxidase (katG), partial CSB-YP374832AVL
CSB-EP374832AVL
CSB-BP374832AVL
CSB-MP374832AVL
CSB-EP374832AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP374833AVL
CSB-EP374833AVL
CSB-BP374833AVL
CSB-MP374833AVL
CSB-EP374833AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Ketol-acid reductoisomerase (ilvC) CSB-YP374834AVL
CSB-EP374834AVL
CSB-BP374834AVL
CSB-MP374834AVL
CSB-EP374834AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. GTPase obg (obg) CSB-YP374835AVL
CSB-EP374835AVL
CSB-BP374835AVL
CSB-MP374835AVL
CSB-EP374835AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Methionine--tRNA ligase (metG), partial CSB-YP374836AVL
CSB-EP374836AVL
CSB-BP374836AVL
CSB-MP374836AVL
CSB-EP374836AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Leucine--tRNA ligase (leuS), partial CSB-YP374837AVL
CSB-EP374837AVL
CSB-BP374837AVL
CSB-MP374837AVL
CSB-EP374837AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 30S ribosomal protein S21 (rpsU) CSB-YP374838AVL
CSB-EP374838AVL
CSB-BP374838AVL
CSB-MP374838AVL
CSB-EP374838AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Indole-3-glycerol phosphate synthase (trpC) CSB-YP374839AVL
CSB-EP374839AVL
CSB-BP374839AVL
CSB-MP374839AVL
CSB-EP374839AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Sec-independent protein translocase protein TatA (tatA), partial CSB-YP374840AVL
CSB-EP374840AVL
CSB-BP374840AVL
CSB-MP374840AVL
CSB-EP374840AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP374841AVL
CSB-EP374841AVL
CSB-BP374841AVL
CSB-MP374841AVL
CSB-EP374841AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP374842AVL
CSB-EP374842AVL
CSB-BP374842AVL
CSB-MP374842AVL
CSB-EP374842AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 30S ribosomal protein S4 (rpsD) CSB-YP374843AVL
CSB-EP374843AVL
CSB-BP374843AVL
CSB-MP374843AVL
CSB-EP374843AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L36 (rpmJ) CSB-YP374844AVL
CSB-EP374844AVL
CSB-BP374844AVL
CSB-MP374844AVL
CSB-EP374844AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L18 (rplR) CSB-YP374845AVL
CSB-EP374845AVL
CSB-BP374845AVL
CSB-MP374845AVL
CSB-EP374845AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L24 (rplX) CSB-YP374846AVL
CSB-EP374846AVL
CSB-BP374846AVL
CSB-MP374846AVL
CSB-EP374846AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L29 (rpmC) CSB-YP374847AVL
CSB-EP374847AVL
CSB-BP374847AVL
CSB-MP374847AVL
CSB-EP374847AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 30S ribosomal protein S3 (rpsC) CSB-YP374848AVL
CSB-EP374848AVL
CSB-BP374848AVL
CSB-MP374848AVL
CSB-EP374848AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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