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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium vanbaalenii Probable DNA ligase (lig), partial CSB-YP376689MOQ
CSB-EP376689MOQ
CSB-BP376689MOQ
CSB-MP376689MOQ
CSB-EP376689MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP376690MOQ
CSB-EP376690MOQ
CSB-BP376690MOQ
CSB-MP376690MOQ
CSB-EP376690MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Ketol-acid reductoisomerase (ilvC) CSB-YP376691MOQ
CSB-EP376691MOQ
CSB-BP376691MOQ
CSB-MP376691MOQ
CSB-EP376691MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP376692MOQ
CSB-EP376692MOQ
CSB-BP376692MOQ
CSB-MP376692MOQ
CSB-EP376692MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP376693MOQ
CSB-EP376693MOQ
CSB-BP376693MOQ
CSB-MP376693MOQ
CSB-EP376693MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Uncharacterized oxidoreductase Mvan_2161 (Mvan_2161) CSB-YP376694MOQ
CSB-EP376694MOQ
CSB-BP376694MOQ
CSB-MP376694MOQ
CSB-EP376694MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP376695MOQ
CSB-EP376695MOQ
CSB-BP376695MOQ
CSB-MP376695MOQ
CSB-EP376695MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Ribosome maturation factor RimM (rimM) CSB-YP376696MOQ
CSB-EP376696MOQ
CSB-BP376696MOQ
CSB-MP376696MOQ
CSB-EP376696MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii UPF0102 protein Mvan_2202 (Mvan_2202) CSB-YP376697MOQ
CSB-EP376697MOQ
CSB-BP376697MOQ
CSB-MP376697MOQ
CSB-EP376697MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Probable malate:quinone oxidoreductase (mqo), partial CSB-YP376698MOQ
CSB-EP376698MOQ
CSB-BP376698MOQ
CSB-MP376698MOQ
CSB-EP376698MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii 30S ribosomal protein S15 (rpsO) CSB-YP376699MOQ
CSB-EP376699MOQ
CSB-BP376699MOQ
CSB-MP376699MOQ
CSB-EP376699MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Dihydrodipicolinate reductase (dapB) CSB-YP376700MOQ
CSB-EP376700MOQ
CSB-BP376700MOQ
CSB-MP376700MOQ
CSB-EP376700MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Protein RecA (recA) CSB-YP376701MOQ
CSB-EP376701MOQ
CSB-BP376701MOQ
CSB-MP376701MOQ
CSB-EP376701MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Regulatory protein recX (recX) CSB-YP376702MOQ
CSB-EP376702MOQ
CSB-BP376702MOQ
CSB-MP376702MOQ
CSB-EP376702MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii LexA repressor (lexA) CSB-YP376703MOQ
CSB-EP376703MOQ
CSB-BP376703MOQ
CSB-MP376703MOQ
CSB-EP376703MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Transcriptional repressor NrdR (nrdR) CSB-YP376704MOQ
CSB-EP376704MOQ
CSB-BP376704MOQ
CSB-MP376704MOQ
CSB-EP376704MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Na (+)/H (+) antiporter nhaA 2, partial CSB-YP376705MOQ
CSB-EP376705MOQ
CSB-BP376705MOQ
CSB-MP376705MOQ
CSB-EP376705MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP376706MOQ
CSB-EP376706MOQ
CSB-BP376706MOQ
CSB-MP376706MOQ
CSB-EP376706MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Probable transcriptional regulatory protein Mvan_2570 (Mvan_2570) CSB-YP376707MOQ
CSB-EP376707MOQ
CSB-BP376707MOQ
CSB-MP376707MOQ
CSB-EP376707MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP376708MOQ
CSB-EP376708MOQ
CSB-BP376708MOQ
CSB-MP376708MOQ
CSB-EP376708MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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