Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Nocardioides sp. 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP376489NFC
CSB-EP376489NFC
CSB-BP376489NFC
CSB-MP376489NFC
CSB-EP376489NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L20 (rplT) CSB-YP376490NFC
CSB-EP376490NFC
CSB-BP376490NFC
CSB-MP376490NFC
CSB-EP376490NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Cobalamin synthase (cobS), partial CSB-YP376491NFC1
CSB-EP376491NFC1
CSB-BP376491NFC1
CSB-MP376491NFC1
CSB-EP376491NFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Putative sporulation transcription regulator WhiA (whiA) CSB-YP376492NFC
CSB-EP376492NFC
CSB-BP376492NFC
CSB-MP376492NFC
CSB-EP376492NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP376493NFC
CSB-EP376493NFC
CSB-BP376493NFC
CSB-MP376493NFC
CSB-EP376493NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Pup--protein ligase (pafA) CSB-YP376494NFC
CSB-EP376494NFC
CSB-BP376494NFC
CSB-MP376494NFC
CSB-EP376494NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP376495NFC
CSB-EP376495NFC
CSB-BP376495NFC
CSB-MP376495NFC
CSB-EP376495NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP376496NFC
CSB-EP376496NFC
CSB-BP376496NFC
CSB-MP376496NFC
CSB-EP376496NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP376497NFC
CSB-EP376497NFC
CSB-BP376497NFC
CSB-MP376497NFC
CSB-EP376497NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP376498NFC
CSB-EP376498NFC
CSB-BP376498NFC
CSB-MP376498NFC
CSB-EP376498NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Ribosome-binding factor A (rbfA) CSB-YP376499NFC
CSB-EP376499NFC
CSB-BP376499NFC
CSB-MP376499NFC
CSB-EP376499NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP376500NFC
CSB-EP376500NFC
CSB-BP376500NFC
CSB-MP376500NFC
CSB-EP376500NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 30S ribosomal protein S2 (rpsB) CSB-YP376501NFC
CSB-EP376501NFC
CSB-BP376501NFC
CSB-MP376501NFC
CSB-EP376501NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Ribonuclease HII (rnhB) CSB-YP376502NFC
CSB-EP376502NFC
CSB-BP376502NFC
CSB-MP376502NFC
CSB-EP376502NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Ribosome maturation factor RimM (rimM) CSB-YP376503NFC
CSB-EP376503NFC
CSB-BP376503NFC
CSB-MP376503NFC
CSB-EP376503NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L28 (rpmB) CSB-YP376504NFC
CSB-EP376504NFC
CSB-BP376504NFC
CSB-MP376504NFC
CSB-EP376504NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Carboxylate-amine ligase Noca_3311 (Noca_3311) CSB-YP376505NFC
CSB-EP376505NFC
CSB-BP376505NFC
CSB-MP376505NFC
CSB-EP376505NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP376506NFC
CSB-EP376506NFC
CSB-BP376506NFC
CSB-MP376506NFC
CSB-EP376506NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. DNA ligase (ligA), partial CSB-YP376507NFC
CSB-EP376507NFC
CSB-BP376507NFC
CSB-MP376507NFC
CSB-EP376507NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L27 (rpmA) CSB-YP376508NFC
CSB-EP376508NFC
CSB-BP376508NFC
CSB-MP376508NFC
CSB-EP376508NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>