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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bungarus candidus Alpha-delta-bungarotoxin-1 (alpha-delta-Bgt-1) CSB-YP375069BPN
CSB-EP375069BPN
CSB-BP375069BPN
CSB-MP375069BPN
CSB-EP375069BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 50S ribosomal protein L28 (rpmB) CSB-YP375070YAK
CSB-EP375070YAK
CSB-BP375070YAK
CSB-MP375070YAK
CSB-EP375070YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Low affinity potassium transport system protein kup (kup), partial CSB-YP375071YAK
CSB-EP375071YAK
CSB-BP375071YAK
CSB-MP375071YAK
CSB-EP375071YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0761 membrane protein YE0031 (YE0031), partial CSB-YP375072YAK1
CSB-EP375072YAK1
CSB-BP375072YAK1
CSB-MP375072YAK1
CSB-EP375072YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B DNA ligase B (ligB), partial CSB-YP375073YAK
CSB-EP375073YAK
CSB-BP375073YAK
CSB-MP375073YAK
CSB-EP375073YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP375074YAK
CSB-EP375074YAK
CSB-BP375074YAK
CSB-MP375074YAK
CSB-EP375074YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP375075YAK
CSB-EP375075YAK
CSB-BP375075YAK
CSB-MP375075YAK
CSB-EP375075YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP375076YAK
CSB-EP375076YAK
CSB-BP375076YAK
CSB-MP375076YAK
CSB-EP375076YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP375077YAK
CSB-EP375077YAK
CSB-BP375077YAK
CSB-MP375077YAK
CSB-EP375077YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B HTH-type transcriptional repressor fabR (fabR) CSB-YP375078YAK
CSB-EP375078YAK
CSB-BP375078YAK
CSB-MP375078YAK
CSB-EP375078YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ketol-acid reductoisomerase (ilvC) CSB-YP375079YAK
CSB-EP375079YAK
CSB-BP375079YAK
CSB-MP375079YAK
CSB-EP375079YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Protein CyaY (cyaY) CSB-YP375080YAK
CSB-EP375080YAK
CSB-BP375080YAK
CSB-MP375080YAK
CSB-EP375080YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP375081YAK
CSB-EP375081YAK
CSB-BP375081YAK
CSB-MP375081YAK
CSB-EP375081YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B sn-glycerol-3-phosphate-binding periplasmic protein ugpB (ugpB) CSB-YP375082YAK
CSB-EP375082YAK
CSB-BP375082YAK
CSB-MP375082YAK
CSB-EP375082YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP375083YAK
CSB-EP375083YAK
CSB-BP375083YAK
CSB-MP375083YAK
CSB-EP375083YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP375084YAK
CSB-EP375084YAK
CSB-BP375084YAK
CSB-MP375084YAK
CSB-EP375084YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Elongation factor Tu 1 (tuf1) CSB-YP375085YAK
CSB-EP375085YAK
CSB-BP375085YAK
CSB-MP375085YAK
CSB-EP375085YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP375086YAK
CSB-EP375086YAK
CSB-BP375086YAK
CSB-MP375086YAK
CSB-EP375086YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP375087YAK
CSB-EP375087YAK
CSB-BP375087YAK
CSB-MP375087YAK
CSB-EP375087YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Threonine/serine transporter TdcC (tdcC), partial CSB-YP375088YAK
CSB-EP375088YAK
CSB-BP375088YAK
CSB-MP375088YAK
CSB-EP375088YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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