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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Azoarcus sp. 30S ribosomal protein S13 (rpsM) CSB-YP375749AVL
CSB-EP375749AVL
CSB-BP375749AVL
CSB-MP375749AVL
CSB-EP375749AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L15 (rplO) CSB-YP375750AVL
CSB-EP375750AVL
CSB-BP375750AVL
CSB-MP375750AVL
CSB-EP375750AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 30S ribosomal protein S5 (rpsE) CSB-YP375751AVL
CSB-EP375751AVL
CSB-BP375751AVL
CSB-MP375751AVL
CSB-EP375751AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L6 (rplF) CSB-YP375752AVL
CSB-EP375752AVL
CSB-BP375752AVL
CSB-MP375752AVL
CSB-EP375752AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 30S ribosomal protein S14 (rpsN) CSB-YP375753AVL
CSB-EP375753AVL
CSB-BP375753AVL
CSB-MP375753AVL
CSB-EP375753AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 30S ribosomal protein S17 (rpsQ) CSB-YP375754AVL
CSB-EP375754AVL
CSB-BP375754AVL
CSB-MP375754AVL
CSB-EP375754AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L2 (rplB) CSB-YP375755AVL
CSB-EP375755AVL
CSB-BP375755AVL
CSB-MP375755AVL
CSB-EP375755AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L3 (rplC) CSB-YP375756AVL
CSB-EP375756AVL
CSB-BP375756AVL
CSB-MP375756AVL
CSB-EP375756AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L10 (rplJ) CSB-YP375757AVL
CSB-EP375757AVL
CSB-BP375757AVL
CSB-MP375757AVL
CSB-EP375757AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Thiamine-phosphate synthase (thiE) CSB-YP375758AVL
CSB-EP375758AVL
CSB-BP375758AVL
CSB-MP375758AVL
CSB-EP375758AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Urease accessory protein UreF (ureF) CSB-YP375759AVL
CSB-EP375759AVL
CSB-BP375759AVL
CSB-MP375759AVL
CSB-EP375759AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Urease subunit alpha (ureC), partial CSB-YP375760AVL
CSB-EP375760AVL
CSB-BP375760AVL
CSB-MP375760AVL
CSB-EP375760AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP375761AVL
CSB-EP375761AVL
CSB-BP375761AVL
CSB-MP375761AVL
CSB-EP375761AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Bifunctional protein GlmU (glmU) CSB-YP375762AVL
CSB-EP375762AVL
CSB-BP375762AVL
CSB-MP375762AVL
CSB-EP375762AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP375763AVL
CSB-EP375763AVL
CSB-BP375763AVL
CSB-MP375763AVL
CSB-EP375763AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Error-prone DNA polymerase (dnaE2), partial CSB-YP375764AVL
CSB-EP375764AVL
CSB-BP375764AVL
CSB-MP375764AVL
CSB-EP375764AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. tRNA (guanine-N (7)-)-methyltransferase CSB-YP375765AVL
CSB-EP375765AVL
CSB-BP375765AVL
CSB-MP375765AVL
CSB-EP375765AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Ribonuclease P protein component (rnpA) CSB-YP375766AVL
CSB-EP375766AVL
CSB-BP375766AVL
CSB-MP375766AVL
CSB-EP375766AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis UPF0301 protein BCG_0069 (BCG_0069) CSB-YP375767MOJ
CSB-EP375767MOJ
CSB-BP375767MOJ
CSB-MP375767MOJ
CSB-EP375767MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Maltokinase (pep2) CSB-YP375768MOJ
CSB-EP375768MOJ
CSB-BP375768MOJ
CSB-MP375768MOJ
CSB-EP375768MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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