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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Azoarcus sp. Chaperone protein DnaK (dnaK), partial CSB-YP375709AVL
CSB-EP375709AVL
CSB-BP375709AVL
CSB-MP375709AVL
CSB-EP375709AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L35 (rpmI) CSB-YP375710AVL
CSB-EP375710AVL
CSB-BP375710AVL
CSB-MP375710AVL
CSB-EP375710AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Dihydrodipicolinate synthase (dapA) CSB-YP375711AVL
CSB-EP375711AVL
CSB-BP375711AVL
CSB-MP375711AVL
CSB-EP375711AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L28 (rpmB) CSB-YP375712AVL
CSB-EP375712AVL
CSB-BP375712AVL
CSB-MP375712AVL
CSB-EP375712AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP375713AVL
CSB-EP375713AVL
CSB-BP375713AVL
CSB-MP375713AVL
CSB-EP375713AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. tRNA (uracil (54)-C (5))-methyltransferase CSB-YP375714AVL
CSB-EP375714AVL
CSB-BP375714AVL
CSB-MP375714AVL
CSB-EP375714AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP375715AVL
CSB-EP375715AVL
CSB-BP375715AVL
CSB-MP375715AVL
CSB-EP375715AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Phosphoglucosamine mutase (glmM) CSB-YP375716AVL
CSB-EP375716AVL
CSB-BP375716AVL
CSB-MP375716AVL
CSB-EP375716AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP375717AVL1
CSB-EP375717AVL1
CSB-BP375717AVL1
CSB-MP375717AVL1
CSB-EP375717AVL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP375718AVL
CSB-EP375718AVL
CSB-BP375718AVL
CSB-MP375718AVL
CSB-EP375718AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP375719AVL
CSB-EP375719AVL
CSB-BP375719AVL
CSB-MP375719AVL
CSB-EP375719AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L31 (rpmE) CSB-YP375720AVL
CSB-EP375720AVL
CSB-BP375720AVL
CSB-MP375720AVL
CSB-EP375720AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UPF0061 protein azo1574 (azo1574), partial CSB-YP375721AVL
CSB-EP375721AVL
CSB-BP375721AVL
CSB-MP375721AVL
CSB-EP375721AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Phosphate acyltransferase (plsX) CSB-YP375722AVL
CSB-EP375722AVL
CSB-BP375722AVL
CSB-MP375722AVL
CSB-EP375722AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. DNA repair protein recO (recO) CSB-YP375723AVL
CSB-EP375723AVL
CSB-BP375723AVL
CSB-MP375723AVL
CSB-EP375723AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP375724AVL
CSB-EP375724AVL
CSB-BP375724AVL
CSB-MP375724AVL
CSB-EP375724AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UPF0391 membrane protein azo1750 (azo1750), partial CSB-YP375725AVL
CSB-EP375725AVL
CSB-BP375725AVL
CSB-MP375725AVL
CSB-EP375725AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UPF0391 membrane protein azo1765 (azo1765), partial CSB-YP375726AVL
CSB-EP375726AVL
CSB-BP375726AVL
CSB-MP375726AVL
CSB-EP375726AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UPF0246 protein azo1887 (azo1887) CSB-YP375727AVL
CSB-EP375727AVL
CSB-BP375727AVL
CSB-MP375727AVL
CSB-EP375727AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP375728AVL
CSB-EP375728AVL
CSB-BP375728AVL
CSB-MP375728AVL
CSB-EP375728AVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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