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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pyrobaculum islandicum Riboflavin kinase (ribK) CSB-YP376949PZW
CSB-EP376949PZW
CSB-BP376949PZW
CSB-MP376949PZW
CSB-EP376949PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Protease HtpX homolog (htpX), partial CSB-YP376950PZW1
CSB-EP376950PZW1
CSB-BP376950PZW1
CSB-MP376950PZW1
CSB-EP376950PZW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Transcription factor E (tfe) CSB-YP376951PZW
CSB-EP376951PZW
CSB-BP376951PZW
CSB-MP376951PZW
CSB-EP376951PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 30S ribosomal protein S2P (rps2p) CSB-YP376952PZW
CSB-EP376952PZW
CSB-BP376952PZW
CSB-MP376952PZW
CSB-EP376952PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum UPF0103 protein Pisl_1105 (Pisl_1105) CSB-YP376953PZW
CSB-EP376953PZW
CSB-BP376953PZW
CSB-MP376953PZW
CSB-EP376953PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum DNA ligase (lig), partial CSB-YP376954PZW
CSB-EP376954PZW
CSB-BP376954PZW
CSB-MP376954PZW
CSB-EP376954PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP376955PZW
CSB-EP376955PZW
CSB-BP376955PZW
CSB-MP376955PZW
CSB-EP376955PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Acylphosphatase (acyP) CSB-YP376956PZW
CSB-EP376956PZW
CSB-BP376956PZW
CSB-MP376956PZW
CSB-EP376956PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Ribonuclease HII (rnhB) CSB-YP376957PZW
CSB-EP376957PZW
CSB-BP376957PZW
CSB-MP376957PZW
CSB-EP376957PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 50S ribosomal protein L30P (rpl30p) CSB-YP376958PZW
CSB-EP376958PZW
CSB-BP376958PZW
CSB-MP376958PZW
CSB-EP376958PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP376959PZW
CSB-EP376959PZW
CSB-BP376959PZW
CSB-MP376959PZW
CSB-EP376959PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Probable non-canonical purine NTP phosphatase (Pisl_1392) CSB-YP376960PZW
CSB-EP376960PZW
CSB-BP376960PZW
CSB-MP376960PZW
CSB-EP376960PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Chromatin protein Cren7 (creN7) CSB-YP376961PZW
CSB-EP376961PZW
CSB-BP376961PZW
CSB-MP376961PZW
CSB-EP376961PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Uracil phosphoribosyltransferase (upp) CSB-YP376962PZW
CSB-EP376962PZW
CSB-BP376962PZW
CSB-MP376962PZW
CSB-EP376962PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 30S ribosomal protein S19P (rps19p) CSB-YP376963PZW
CSB-EP376963PZW
CSB-BP376963PZW
CSB-MP376963PZW
CSB-EP376963PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Arginine decarboxylase proenzyme (Pisl_1714) CSB-YP376964PZW
CSB-EP376964PZW
CSB-BP376964PZW
CSB-MP376964PZW
CSB-EP376964PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 3-dehydroquinate synthase (aroB) CSB-YP376965PZW
CSB-EP376965PZW
CSB-BP376965PZW
CSB-MP376965PZW
CSB-EP376965PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP376966PZW
CSB-EP376966PZW
CSB-BP376966PZW
CSB-MP376966PZW
CSB-EP376966PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 50S ribosomal protein L18P (rpl18p) CSB-YP376967PZW
CSB-EP376967PZW
CSB-BP376967PZW
CSB-MP376967PZW
CSB-EP376967PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog (Pisl_1893) CSB-YP376968PZW
CSB-EP376968PZW
CSB-BP376968PZW
CSB-MP376968PZW
CSB-EP376968PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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