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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Psychromonas ingrahamii Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP376609PZS
CSB-EP376609PZS
CSB-BP376609PZS
CSB-MP376609PZS
CSB-EP376609PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Cell division protein ZapC (zapC) CSB-YP376610PZS
CSB-EP376610PZS
CSB-BP376610PZS
CSB-MP376610PZS
CSB-EP376610PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii DNA ligase (ligA), partial CSB-YP376611PZS
CSB-EP376611PZS
CSB-BP376611PZS
CSB-MP376611PZS
CSB-EP376611PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 30S ribosomal protein S4 A (rpsD1) CSB-YP376612PZS
CSB-EP376612PZS
CSB-BP376612PZS
CSB-MP376612PZS
CSB-EP376612PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii DNA-directed RNA polymerase subunit alpha 1 (rpoA1) CSB-YP376613PZS
CSB-EP376613PZS
CSB-BP376613PZS
CSB-MP376613PZS
CSB-EP376613PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii UPF0265 protein Ping_2721 (Ping_2721) CSB-YP376614PZS
CSB-EP376614PZS
CSB-BP376614PZS
CSB-MP376614PZS
CSB-EP376614PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP376615PZS
CSB-EP376615PZS
CSB-BP376615PZS
CSB-MP376615PZS
CSB-EP376615PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP376616PZS
CSB-EP376616PZS
CSB-BP376616PZS
CSB-MP376616PZS
CSB-EP376616PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 60 kDa chaperonin 3 (groL3), partial CSB-YP376617PZS
CSB-EP376617PZS
CSB-BP376617PZS
CSB-MP376617PZS
CSB-EP376617PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP376618PZS
CSB-EP376618PZS
CSB-BP376618PZS
CSB-MP376618PZS
CSB-EP376618PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Phosphopentomutase (deoB) CSB-YP376619PZS
CSB-EP376619PZS
CSB-BP376619PZS
CSB-MP376619PZS
CSB-EP376619PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP376620PZS
CSB-EP376620PZS
CSB-BP376620PZS
CSB-MP376620PZS
CSB-EP376620PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Ribonuclease HII (rnhB) CSB-YP376621PZS
CSB-EP376621PZS
CSB-BP376621PZS
CSB-MP376621PZS
CSB-EP376621PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP376622PZS
CSB-EP376622PZS
CSB-BP376622PZS
CSB-MP376622PZS
CSB-EP376622PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP376623PZS
CSB-EP376623PZS
CSB-BP376623PZS
CSB-MP376623PZS
CSB-EP376623PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Urease accessory protein UreD (ureD) CSB-YP376624PZS
CSB-EP376624PZS
CSB-BP376624PZS
CSB-MP376624PZS
CSB-EP376624PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Urease subunit beta (ureB) CSB-YP376625PZS
CSB-EP376625PZS
CSB-BP376625PZS
CSB-MP376625PZS
CSB-EP376625PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Urease accessory protein UreF (ureF) CSB-YP376626PZS
CSB-EP376626PZS
CSB-BP376626PZS
CSB-MP376626PZS
CSB-EP376626PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP376627PZS
CSB-EP376627PZS
CSB-BP376627PZS
CSB-MP376627PZS
CSB-EP376627PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP376628PZS
CSB-EP376628PZS
CSB-BP376628PZS
CSB-MP376628PZS
CSB-EP376628PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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