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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Psychromonas ingrahamii Peptidyl-tRNA hydrolase (pth) CSB-YP376569PZS
CSB-EP376569PZS
CSB-BP376569PZS
CSB-MP376569PZS
CSB-EP376569PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Outer-membrane lipoprotein lolB (lolB) CSB-YP376570PZS
CSB-EP376570PZS
CSB-BP376570PZS
CSB-MP376570PZS
CSB-EP376570PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Chaperone protein DnaK (dnaK), partial CSB-YP376571PZS
CSB-EP376571PZS
CSB-BP376571PZS
CSB-MP376571PZS
CSB-EP376571PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Chaperone protein DnaJ (dnaJ) CSB-YP376572PZS
CSB-EP376572PZS
CSB-BP376572PZS
CSB-MP376572PZS
CSB-EP376572PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii UPF0276 protein Ping_0944 (Ping_0944) CSB-YP376573PZS
CSB-EP376573PZS
CSB-BP376573PZS
CSB-MP376573PZS
CSB-EP376573PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Chorismate synthase (aroC) CSB-YP376574PZS
CSB-EP376574PZS
CSB-BP376574PZS
CSB-MP376574PZS
CSB-EP376574PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP376575PZS
CSB-EP376575PZS
CSB-BP376575PZS
CSB-MP376575PZS
CSB-EP376575PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Probable intracellular septation protein A (Ping_1054), partial CSB-YP376576PZS1
CSB-EP376576PZS1
CSB-BP376576PZS1
CSB-MP376576PZS1
CSB-EP376576PZS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP376577PZS
CSB-EP376577PZS
CSB-BP376577PZS
CSB-MP376577PZS
CSB-EP376577PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii UvrABC system protein B (uvrB), partial CSB-YP376578PZS
CSB-EP376578PZS
CSB-BP376578PZS
CSB-MP376578PZS
CSB-EP376578PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Phosphate acyltransferase (plsX) CSB-YP376579PZS
CSB-EP376579PZS
CSB-BP376579PZS
CSB-MP376579PZS
CSB-EP376579PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP376580PZS
CSB-EP376580PZS
CSB-BP376580PZS
CSB-MP376580PZS
CSB-EP376580PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Cell division protein FtsL (ftsL), partial CSB-YP376581PZS
CSB-EP376581PZS
CSB-BP376581PZS
CSB-MP376581PZS
CSB-EP376581PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Protein translocase subunit SecA (secA), partial CSB-YP376582PZS
CSB-EP376582PZS
CSB-BP376582PZS
CSB-MP376582PZS
CSB-EP376582PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii GTPase Der (der) CSB-YP376583PZS
CSB-EP376583PZS
CSB-BP376583PZS
CSB-MP376583PZS
CSB-EP376583PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Leucine--tRNA ligase (leuS), partial CSB-YP376584PZS
CSB-EP376584PZS
CSB-BP376584PZS
CSB-MP376584PZS
CSB-EP376584PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP376585PZS
CSB-EP376585PZS
CSB-BP376585PZS
CSB-MP376585PZS
CSB-EP376585PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Autonomous glycyl radical cofactor (grcA) CSB-YP376586PZS
CSB-EP376586PZS
CSB-BP376586PZS
CSB-MP376586PZS
CSB-EP376586PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Integration host factor subunit alpha (ihfA) CSB-YP376587PZS
CSB-EP376587PZS
CSB-BP376587PZS
CSB-MP376587PZS
CSB-EP376587PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii N utilization substance protein B homolog (nusB) CSB-YP376588PZS
CSB-EP376588PZS
CSB-BP376588PZS
CSB-MP376588PZS
CSB-EP376588PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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