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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arthrobacter aurescens Octanoyltransferase (lipB) CSB-YP376769AUZ
CSB-EP376769AUZ
CSB-BP376769AUZ
CSB-MP376769AUZ
CSB-EP376769AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP376770AUZ
CSB-EP376770AUZ
CSB-BP376770AUZ
CSB-MP376770AUZ
CSB-EP376770AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP376771AUZ
CSB-EP376771AUZ
CSB-BP376771AUZ
CSB-MP376771AUZ
CSB-EP376771AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens UvrABC system protein B (uvrB), partial CSB-YP376772AUZ
CSB-EP376772AUZ
CSB-BP376772AUZ
CSB-MP376772AUZ
CSB-EP376772AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Putative sporulation transcription regulator WhiA (whiA) CSB-YP376773AUZ
CSB-EP376773AUZ
CSB-BP376773AUZ
CSB-MP376773AUZ
CSB-EP376773AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP376774AUZ
CSB-EP376774AUZ
CSB-BP376774AUZ
CSB-MP376774AUZ
CSB-EP376774AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Proteasome-associated ATPase (arc), partial CSB-YP376775AUZ
CSB-EP376775AUZ
CSB-BP376775AUZ
CSB-MP376775AUZ
CSB-EP376775AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Probable rRNA maturation factor (AAur_2230) CSB-YP376776AUZ
CSB-EP376776AUZ
CSB-BP376776AUZ
CSB-MP376776AUZ
CSB-EP376776AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens 30S ribosomal protein S20 (rpsT) CSB-YP376777AUZ
CSB-EP376777AUZ
CSB-BP376777AUZ
CSB-MP376777AUZ
CSB-EP376777AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens 30S ribosomal protein S4 (rpsD) CSB-YP376778AUZ
CSB-EP376778AUZ
CSB-BP376778AUZ
CSB-MP376778AUZ
CSB-EP376778AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Probable transcriptional regulatory protein AAur_2300 (AAur_2300) CSB-YP376779AUZ
CSB-EP376779AUZ
CSB-BP376779AUZ
CSB-MP376779AUZ
CSB-EP376779AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP376780AUZ
CSB-EP376780AUZ
CSB-BP376780AUZ
CSB-MP376780AUZ
CSB-EP376780AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP376781AUZ
CSB-EP376781AUZ
CSB-BP376781AUZ
CSB-MP376781AUZ
CSB-EP376781AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Ribosome maturation factor RimM (rimM) CSB-YP376782AUZ
CSB-EP376782AUZ
CSB-BP376782AUZ
CSB-MP376782AUZ
CSB-EP376782AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens D-alanine--D-alanine ligase (ddl) CSB-YP376783AUZ
CSB-EP376783AUZ
CSB-BP376783AUZ
CSB-MP376783AUZ
CSB-EP376783AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP376784AUZ
CSB-EP376784AUZ
CSB-BP376784AUZ
CSB-MP376784AUZ
CSB-EP376784AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens Probable malate:quinone oxidoreductase (mqo), partial CSB-YP376785AUZ
CSB-EP376785AUZ
CSB-BP376785AUZ
CSB-MP376785AUZ
CSB-EP376785AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens SsrA-binding protein (smpB) CSB-YP376786AUZ
CSB-EP376786AUZ
CSB-BP376786AUZ
CSB-MP376786AUZ
CSB-EP376786AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens UPF0182 protein AAur_2732 (AAur_2732), partial CSB-YP376787AUZ
CSB-EP376787AUZ
CSB-BP376787AUZ
CSB-MP376787AUZ
CSB-EP376787AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter aurescens 60 kDa chaperonin 2 (groL2), partial CSB-YP376788AUZ
CSB-EP376788AUZ
CSB-BP376788AUZ
CSB-MP376788AUZ
CSB-EP376788AUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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