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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium vanbaalenii Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP376709MOQ
CSB-EP376709MOQ
CSB-BP376709MOQ
CSB-MP376709MOQ
CSB-EP376709MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Alanine--tRNA ligase (alaS), partial CSB-YP376710MOQ
CSB-EP376710MOQ
CSB-BP376710MOQ
CSB-MP376710MOQ
CSB-EP376710MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii 3-dehydroquinate synthase (aroB) CSB-YP376711MOQ
CSB-EP376711MOQ
CSB-BP376711MOQ
CSB-MP376711MOQ
CSB-EP376711MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii N utilization substance protein B homolog (nusB) CSB-YP376712MOQ
CSB-EP376712MOQ
CSB-BP376712MOQ
CSB-MP376712MOQ
CSB-EP376712MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Aspartate carbamoyltransferase (pyrB) CSB-YP376713MOQ
CSB-EP376713MOQ
CSB-BP376713MOQ
CSB-MP376713MOQ
CSB-EP376713MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP376714MOQ
CSB-EP376714MOQ
CSB-BP376714MOQ
CSB-MP376714MOQ
CSB-EP376714MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP376715MOQ
CSB-EP376715MOQ
CSB-BP376715MOQ
CSB-MP376715MOQ
CSB-EP376715MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP376716MOQ
CSB-EP376716MOQ
CSB-BP376716MOQ
CSB-MP376716MOQ
CSB-EP376716MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii UPF0042 nucleotide-binding protein Mvan_2698 (Mvan_2698) CSB-YP376717MOQ
CSB-EP376717MOQ
CSB-BP376717MOQ
CSB-MP376717MOQ
CSB-EP376717MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Phosphoglycerate kinase (pgk) CSB-YP376718MOQ
CSB-EP376718MOQ
CSB-BP376718MOQ
CSB-MP376718MOQ
CSB-EP376718MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii UPF0353 protein Mvan_2751 (Mvan_2751), partial CSB-YP376719MOQ1
CSB-EP376719MOQ1
CSB-BP376719MOQ1
CSB-MP376719MOQ1
CSB-EP376719MOQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Cyanate hydratase (cynS) CSB-YP376720MOQ
CSB-EP376720MOQ
CSB-BP376720MOQ
CSB-MP376720MOQ
CSB-EP376720MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (Mvan_2789) CSB-YP376721MOQ
CSB-EP376721MOQ
CSB-BP376721MOQ
CSB-MP376721MOQ
CSB-EP376721MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Histidinol-phosphate aminotransferase (hisC) CSB-YP376722MOQ
CSB-EP376722MOQ
CSB-BP376722MOQ
CSB-MP376722MOQ
CSB-EP376722MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium vanbaalenii Phosphoribosyl isomerase A (priA) CSB-YP376723MOQ
CSB-EP376723MOQ
CSB-BP376723MOQ
CSB-MP376723MOQ
CSB-EP376723MOQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ubiquitin-conjugating enzyme E2 S-A (ube2s-a) CSB-YP376724XBE
CSB-EP376724XBE
CSB-BP376724XBE
CSB-MP376724XBE
CSB-EP376724XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human G antigen 12B/C/D/E (GAGE12B) CSB-YP376725HU
CSB-EP376725HU
CSB-BP376725HU
CSB-MP376725HU
CSB-EP376725HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Enolase (eno) CSB-YP376726MOO
CSB-EP376726MOO
CSB-BP376726MOO
CSB-MP376726MOO
CSB-EP376726MOO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia turicatae Probable rRNA maturation factor (BT0060) CSB-YP376727BPC
CSB-EP376727BPC
CSB-BP376727BPC
CSB-MP376727BPC
CSB-EP376727BPC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia turicatae V-type ATP synthase beta chain (atpB) CSB-YP376728BPC
CSB-EP376728BPC
CSB-BP376728BPC
CSB-MP376728BPC
CSB-EP376728BPC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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