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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Shewanella sp. Shikimate kinase (aroK) CSB-YP376909STT
CSB-EP376909STT
CSB-BP376909STT
CSB-MP376909STT
CSB-EP376909STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. CinA-like protein (Sputw3181_3863) CSB-YP376910STT
CSB-EP376910STT
CSB-BP376910STT
CSB-MP376910STT
CSB-EP376910STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP376911STT
CSB-EP376911STT
CSB-BP376911STT
CSB-MP376911STT
CSB-EP376911STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP376912STT
CSB-EP376912STT
CSB-BP376912STT
CSB-MP376912STT
CSB-EP376912STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. ATP synthase epsilon chain (atpC) CSB-YP376913STT
CSB-EP376913STT
CSB-BP376913STT
CSB-MP376913STT
CSB-EP376913STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. ATP synthase gamma chain (atpG) CSB-YP376914STT
CSB-EP376914STT
CSB-BP376914STT
CSB-MP376914STT
CSB-EP376914STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. ATP synthase subunit c (atpE), partial CSB-YP376915STT1
CSB-EP376915STT1
CSB-BP376915STT1
CSB-MP376915STT1
CSB-EP376915STT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. tRNA modification GTPase MnmE (mnmE) CSB-YP376916STT
CSB-EP376916STT
CSB-BP376916STT
CSB-MP376916STT
CSB-EP376916STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 50S ribosomal protein L34 (rpmH) CSB-YP376917STT
CSB-EP376917STT
CSB-BP376917STT
CSB-MP376917STT
CSB-EP376917STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Homoserine kinase (thrB) CSB-YP376918PZW
CSB-EP376918PZW
CSB-BP376918PZW
CSB-MP376918PZW
CSB-EP376918PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP376919PZW
CSB-EP376919PZW
CSB-BP376919PZW
CSB-MP376919PZW
CSB-EP376919PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Undecaprenyl-diphosphatase (uppP), partial CSB-YP376920PZW1
CSB-EP376920PZW1
CSB-BP376920PZW1
CSB-MP376920PZW1
CSB-EP376920PZW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Phosphoenolpyruvate carboxylase (ppcA) CSB-YP376921PZW
CSB-EP376921PZW
CSB-BP376921PZW
CSB-MP376921PZW
CSB-EP376921PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Uncharacterized DAPA-like lyase Pisl_0309 (dapAL) CSB-YP376922PZW
CSB-EP376922PZW
CSB-BP376922PZW
CSB-MP376922PZW
CSB-EP376922PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum GTP cyclohydrolase III (gch3) CSB-YP376923PZW
CSB-EP376923PZW
CSB-BP376923PZW
CSB-MP376923PZW
CSB-EP376923PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Glutamyl-tRNA (Gln) amidotransferase subunit E, partial CSB-YP376924PZW
CSB-EP376924PZW
CSB-BP376924PZW
CSB-MP376924PZW
CSB-EP376924PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 30S ribosomal protein S27e (rps27e) CSB-YP376925PZW
CSB-EP376925PZW
CSB-BP376925PZW
CSB-MP376925PZW
CSB-EP376925PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum DNA-binding protein Pisl_0441 (Pisl_0441) CSB-YP376926PZW
CSB-EP376926PZW
CSB-BP376926PZW
CSB-MP376926PZW
CSB-EP376926PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 50S ribosomal protein L31e (rpl31e) CSB-YP376927PZW
CSB-EP376927PZW
CSB-BP376927PZW
CSB-MP376927PZW
CSB-EP376927PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum 50S ribosomal protein L40e (rpl40e) CSB-YP376928PZW
CSB-EP376928PZW
CSB-BP376928PZW
CSB-MP376928PZW
CSB-EP376928PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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