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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nocardioides sp. Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP377809NFC
CSB-EP377809NFC
CSB-BP377809NFC
CSB-MP377809NFC
CSB-EP377809NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Proteasome-associated ATPase (arc), partial CSB-YP377810NFC
CSB-EP377810NFC
CSB-BP377810NFC
CSB-MP377810NFC
CSB-EP377810NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Proteasome subunit alpha (prcA) CSB-YP377811NFC
CSB-EP377811NFC
CSB-BP377811NFC
CSB-MP377811NFC
CSB-EP377811NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Probable DNA ligase (lig), partial CSB-YP377812NFC
CSB-EP377812NFC
CSB-BP377812NFC
CSB-MP377812NFC
CSB-EP377812NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Indole-3-glycerol phosphate synthase (trpC) CSB-YP377813NFC
CSB-EP377813NFC
CSB-BP377813NFC
CSB-MP377813NFC
CSB-EP377813NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Cell division protein SepF (sepF) CSB-YP377814NFC
CSB-EP377814NFC
CSB-BP377814NFC
CSB-MP377814NFC
CSB-EP377814NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP377815NFC
CSB-EP377815NFC
CSB-BP377815NFC
CSB-MP377815NFC
CSB-EP377815NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Anthranilate phosphoribosyltransferase (trpD) CSB-YP377816NFC
CSB-EP377816NFC
CSB-BP377816NFC
CSB-MP377816NFC
CSB-EP377816NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Quinolinate synthase A (nadA) CSB-YP377817NFC
CSB-EP377817NFC
CSB-BP377817NFC
CSB-MP377817NFC
CSB-EP377817NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Proline--tRNA ligase (proS), partial CSB-YP377818NFC
CSB-EP377818NFC
CSB-BP377818NFC
CSB-MP377818NFC
CSB-EP377818NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Uridylate kinase (pyrH) CSB-YP377819NFC
CSB-EP377819NFC
CSB-BP377819NFC
CSB-MP377819NFC
CSB-EP377819NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. UPF0102 protein Noca_3248 (Noca_3248) CSB-YP377820NFC
CSB-EP377820NFC
CSB-BP377820NFC
CSB-MP377820NFC
CSB-EP377820NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L19 (rplS) CSB-YP377821NFC
CSB-EP377821NFC
CSB-BP377821NFC
CSB-MP377821NFC
CSB-EP377821NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Phosphopantetheine adenylyltransferase (coaD) CSB-YP377822NFC
CSB-EP377822NFC
CSB-BP377822NFC
CSB-MP377822NFC
CSB-EP377822NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP377823NFC
CSB-EP377823NFC
CSB-BP377823NFC
CSB-MP377823NFC
CSB-EP377823NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Dihydroxy-acid dehydratase (ilvD), partial CSB-YP377824NFC
CSB-EP377824NFC
CSB-BP377824NFC
CSB-MP377824NFC
CSB-EP377824NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP377825NFC
CSB-EP377825NFC
CSB-BP377825NFC
CSB-MP377825NFC
CSB-EP377825NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L21 (rplU) CSB-YP377826NFC
CSB-EP377826NFC
CSB-BP377826NFC
CSB-MP377826NFC
CSB-EP377826NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP377827NFC
CSB-EP377827NFC
CSB-BP377827NFC
CSB-MP377827NFC
CSB-EP377827NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Homoserine O-acetyltransferase (metX) CSB-YP377828NFC
CSB-EP377828NFC
CSB-BP377828NFC
CSB-MP377828NFC
CSB-EP377828NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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