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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella amazonensis Gamma-glutamyl phosphate reductase (proA) CSB-YP377749STK
CSB-EP377749STK
CSB-BP377749STK
CSB-MP377749STK
CSB-EP377749STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Na (+)-translocating NADH-quinone reductase subunit A CSB-YP377750STK
CSB-EP377750STK
CSB-BP377750STK
CSB-MP377750STK
CSB-EP377750STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP377751STK
CSB-EP377751STK
CSB-BP377751STK
CSB-MP377751STK
CSB-EP377751STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP377752STK
CSB-EP377752STK
CSB-BP377752STK
CSB-MP377752STK
CSB-EP377752STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Outer-membrane lipoprotein lolB (lolB) CSB-YP377753STK
CSB-EP377753STK
CSB-BP377753STK
CSB-MP377753STK
CSB-EP377753STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Peptidyl-tRNA hydrolase (pth) CSB-YP377754STK
CSB-EP377754STK
CSB-BP377754STK
CSB-MP377754STK
CSB-EP377754STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP377755STK
CSB-EP377755STK
CSB-BP377755STK
CSB-MP377755STK
CSB-EP377755STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribose-5-phosphate isomerase A (rpiA) CSB-YP377756STK
CSB-EP377756STK
CSB-BP377756STK
CSB-MP377756STK
CSB-EP377756STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP377757STK
CSB-EP377757STK
CSB-BP377757STK
CSB-MP377757STK
CSB-EP377757STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis GTPase obg (obg) CSB-YP377758STK
CSB-EP377758STK
CSB-BP377758STK
CSB-MP377758STK
CSB-EP377758STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP377759STK
CSB-EP377759STK
CSB-BP377759STK
CSB-MP377759STK
CSB-EP377759STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Dihydroorotase (pyrC) CSB-YP377760STK
CSB-EP377760STK
CSB-BP377760STK
CSB-MP377760STK
CSB-EP377760STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP377761STK
CSB-EP377761STK
CSB-BP377761STK
CSB-MP377761STK
CSB-EP377761STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S18 (rpsR) CSB-YP377762STK
CSB-EP377762STK
CSB-BP377762STK
CSB-MP377762STK
CSB-EP377762STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 30S ribosomal protein S6 (rpsF) CSB-YP377763STK
CSB-EP377763STK
CSB-BP377763STK
CSB-MP377763STK
CSB-EP377763STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0042 nucleotide-binding protein Sama_3091 (Sama_3091) CSB-YP377764STK
CSB-EP377764STK
CSB-BP377764STK
CSB-MP377764STK
CSB-EP377764STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 60 kDa chaperonin (groL), partial CSB-YP377765STK
CSB-EP377765STK
CSB-BP377765STK
CSB-MP377765STK
CSB-EP377765STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosomal protein S6 modification protein 2 (rimK2) CSB-YP377766STK
CSB-EP377766STK
CSB-BP377766STK
CSB-MP377766STK
CSB-EP377766STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Sec-independent protein translocase protein TatA (tatA), partial CSB-YP377767STK
CSB-EP377767STK
CSB-BP377767STK
CSB-MP377767STK
CSB-EP377767STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis ATP-dependent RNA helicase rhlB (rhlB) CSB-YP377768STK
CSB-EP377768STK
CSB-BP377768STK
CSB-MP377768STK
CSB-EP377768STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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