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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella amazonensis Probable septum site-determining protein MinC (minC) CSB-YP377729STK
CSB-EP377729STK
CSB-BP377729STK
CSB-MP377729STK
CSB-EP377729STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Histidinol-phosphate aminotransferase (hisC) CSB-YP377730STK
CSB-EP377730STK
CSB-BP377730STK
CSB-MP377730STK
CSB-EP377730STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP377731STK
CSB-EP377731STK
CSB-BP377731STK
CSB-MP377731STK
CSB-EP377731STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP377732STK
CSB-EP377732STK
CSB-BP377732STK
CSB-MP377732STK
CSB-EP377732STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis UPF0502 protein Sama_1967 (Sama_1967) CSB-YP377733STK
CSB-EP377733STK
CSB-BP377733STK
CSB-MP377733STK
CSB-EP377733STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis 50S ribosomal protein L32 (rpmF) CSB-YP377734STK
CSB-EP377734STK
CSB-BP377734STK
CSB-MP377734STK
CSB-EP377734STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Methionine--tRNA ligase (metG), partial CSB-YP377735STK
CSB-EP377735STK
CSB-BP377735STK
CSB-MP377735STK
CSB-EP377735STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Putative arginyl-tRNA--protein transferase (ate) CSB-YP377736STK
CSB-EP377736STK
CSB-BP377736STK
CSB-MP377736STK
CSB-EP377736STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis High frequency lysogenization protein HflD homolog (hflD) CSB-YP377737STK
CSB-EP377737STK
CSB-BP377737STK
CSB-MP377737STK
CSB-EP377737STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP377738STK
CSB-EP377738STK
CSB-BP377738STK
CSB-MP377738STK
CSB-EP377738STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Probable intracellular septation protein A (Sama_2137), partial CSB-YP377739STK1
CSB-EP377739STK1
CSB-BP377739STK1
CSB-MP377739STK1
CSB-EP377739STK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP377740STK
CSB-EP377740STK
CSB-BP377740STK
CSB-MP377740STK
CSB-EP377740STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP377741STK
CSB-EP377741STK
CSB-BP377741STK
CSB-MP377741STK
CSB-EP377741STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Probable phosphatase Sama_2233 (Sama_2233) CSB-YP377742STK
CSB-EP377742STK
CSB-BP377742STK
CSB-MP377742STK
CSB-EP377742STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Flagellar L-ring protein (flgH) CSB-YP377743STK
CSB-EP377743STK
CSB-BP377743STK
CSB-MP377743STK
CSB-EP377743STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP377744STK
CSB-EP377744STK
CSB-BP377744STK
CSB-MP377744STK
CSB-EP377744STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis SsrA-binding protein (smpB) CSB-YP377745STK
CSB-EP377745STK
CSB-BP377745STK
CSB-MP377745STK
CSB-EP377745STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA sulfurtransferase (thiI) CSB-YP377746STK
CSB-EP377746STK
CSB-BP377746STK
CSB-MP377746STK
CSB-EP377746STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP377747STK
CSB-EP377747STK
CSB-BP377747STK
CSB-MP377747STK
CSB-EP377747STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Chaperone protein DnaJ (dnaJ) CSB-YP377748STK
CSB-EP377748STK
CSB-BP377748STK
CSB-MP377748STK
CSB-EP377748STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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