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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidovorax citrulli 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP378109AUB
CSB-EP378109AUB
CSB-BP378109AUB
CSB-MP378109AUB
CSB-EP378109AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Ribosome maturation factor RimP (rimP) CSB-YP378110AUB
CSB-EP378110AUB
CSB-BP378110AUB
CSB-MP378110AUB
CSB-EP378110AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli UPF0502 protein Aave_3438 (Aave_3438) CSB-YP378111AUB
CSB-EP378111AUB
CSB-BP378111AUB
CSB-MP378111AUB
CSB-EP378111AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli UPF0271 protein Aave_3513 (Aave_3513) CSB-YP378112AUB
CSB-EP378112AUB
CSB-BP378112AUB
CSB-MP378112AUB
CSB-EP378112AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Urease subunit alpha (ureC), partial CSB-YP378113AUB
CSB-EP378113AUB
CSB-BP378113AUB
CSB-MP378113AUB
CSB-EP378113AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Non-canonical purine NTP pyrophosphatase (Aave_3578) CSB-YP378114AUB
CSB-EP378114AUB
CSB-BP378114AUB
CSB-MP378114AUB
CSB-EP378114AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Heat-inducible transcription repressor HrcA (hrcA) CSB-YP378115AUB
CSB-EP378115AUB
CSB-BP378115AUB
CSB-MP378115AUB
CSB-EP378115AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli 50S ribosomal protein L25 (rplY) CSB-YP378116AUB
CSB-EP378116AUB
CSB-BP378116AUB
CSB-MP378116AUB
CSB-EP378116AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP378117AUB1
CSB-EP378117AUB1
CSB-BP378117AUB1
CSB-MP378117AUB1
CSB-EP378117AUB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Proline--tRNA ligase (proS), partial CSB-YP378118AUB
CSB-EP378118AUB
CSB-BP378118AUB
CSB-MP378118AUB
CSB-EP378118AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Glutamate 5-kinase (proB) CSB-YP378119AUB
CSB-EP378119AUB
CSB-BP378119AUB
CSB-MP378119AUB
CSB-EP378119AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli 50S ribosomal protein L21 (rplU) CSB-YP378120AUB
CSB-EP378120AUB
CSB-BP378120AUB
CSB-MP378120AUB
CSB-EP378120AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Serine--tRNA ligase (serS) CSB-YP378121AUB
CSB-EP378121AUB
CSB-BP378121AUB
CSB-MP378121AUB
CSB-EP378121AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Pantothenate synthetase (panC) CSB-YP378122AUB
CSB-EP378122AUB
CSB-BP378122AUB
CSB-MP378122AUB
CSB-EP378122AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli UPF0753 protein Aave_4033 (Aave_4033), partial CSB-YP378123AUB
CSB-EP378123AUB
CSB-BP378123AUB
CSB-MP378123AUB
CSB-EP378123AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli 30S ribosomal protein S9 (rpsI) CSB-YP378124AUB
CSB-EP378124AUB
CSB-BP378124AUB
CSB-MP378124AUB
CSB-EP378124AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Type III pantothenate kinase (coaX) CSB-YP378125AUB
CSB-EP378125AUB
CSB-BP378125AUB
CSB-MP378125AUB
CSB-EP378125AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP378126AUB
CSB-EP378126AUB
CSB-BP378126AUB
CSB-MP378126AUB
CSB-EP378126AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP378127AUB
CSB-EP378127AUB
CSB-BP378127AUB
CSB-MP378127AUB
CSB-EP378127AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax citrulli D-tyrosyl-tRNA (Tyr) deacylase CSB-YP378128AUB
CSB-EP378128AUB
CSB-BP378128AUB
CSB-MP378128AUB
CSB-EP378128AUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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