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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pyogenes serotype M5 Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP381653SUQ
CSB-EP381653SUQ
CSB-BP381653SUQ
CSB-MP381653SUQ
CSB-EP381653SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Ferredoxin--NADP reductase (SpyM51151) CSB-YP381654SUQ
CSB-EP381654SUQ
CSB-BP381654SUQ
CSB-MP381654SUQ
CSB-EP381654SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Ribosome maturation factor RimM (rimM) CSB-YP381655SUQ
CSB-EP381655SUQ
CSB-BP381655SUQ
CSB-MP381655SUQ
CSB-EP381655SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Aspartate carbamoyltransferase (pyrB) CSB-YP381656SUQ
CSB-EP381656SUQ
CSB-BP381656SUQ
CSB-MP381656SUQ
CSB-EP381656SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L21 (rplU) CSB-YP381657SUQ
CSB-EP381657SUQ
CSB-BP381657SUQ
CSB-MP381657SUQ
CSB-EP381657SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Chorismate synthase (aroC) CSB-YP381658SUQ
CSB-EP381658SUQ
CSB-BP381658SUQ
CSB-MP381658SUQ
CSB-EP381658SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Cytidylate kinase (cmk) CSB-YP381659SUQ
CSB-EP381659SUQ
CSB-BP381659SUQ
CSB-MP381659SUQ
CSB-EP381659SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP381660SUQ
CSB-EP381660SUQ
CSB-BP381660SUQ
CSB-MP381660SUQ
CSB-EP381660SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 ATP synthase epsilon chain (atpC) CSB-YP381661SUQ
CSB-EP381661SUQ
CSB-BP381661SUQ
CSB-MP381661SUQ
CSB-EP381661SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 DNA ligase (ligA), partial CSB-YP381662SUQ
CSB-EP381662SUQ
CSB-BP381662SUQ
CSB-MP381662SUQ
CSB-EP381662SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Septation ring formation regulator EzrA (ezrA), partial CSB-YP381663SUQ
CSB-EP381663SUQ
CSB-BP381663SUQ
CSB-MP381663SUQ
CSB-EP381663SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L31 type B (rpmE2) CSB-YP381664SUQ
CSB-EP381664SUQ
CSB-BP381664SUQ
CSB-MP381664SUQ
CSB-EP381664SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Peptide chain release factor 2 (prfB) CSB-YP381665SUQ
CSB-EP381665SUQ
CSB-BP381665SUQ
CSB-MP381665SUQ
CSB-EP381665SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP381666SUQ
CSB-EP381666SUQ
CSB-BP381666SUQ
CSB-MP381666SUQ
CSB-EP381666SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 SsrA-binding protein (smpB) CSB-YP381667SUQ
CSB-EP381667SUQ
CSB-BP381667SUQ
CSB-MP381667SUQ
CSB-EP381667SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Probable rRNA maturation factor (SpyM51480) CSB-YP381668SUQ
CSB-EP381668SUQ
CSB-BP381668SUQ
CSB-MP381668SUQ
CSB-EP381668SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Protein nrdI (nrdI) CSB-YP381669SUQ
CSB-EP381669SUQ
CSB-BP381669SUQ
CSB-MP381669SUQ
CSB-EP381669SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Uracil phosphoribosyltransferase (upp) CSB-YP381670SUQ
CSB-EP381670SUQ
CSB-BP381670SUQ
CSB-MP381670SUQ
CSB-EP381670SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Segregation and condensation protein B (scpB) CSB-YP381671SUQ
CSB-EP381671SUQ
CSB-BP381671SUQ
CSB-MP381671SUQ
CSB-EP381671SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Tyrosine recombinase XerD-like (SpyM51551) CSB-YP381672SUQ
CSB-EP381672SUQ
CSB-BP381672SUQ
CSB-MP381672SUQ
CSB-EP381672SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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