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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactococcus lactis subsp. cremoris Protein translocase subunit SecA (secA), partial CSB-YP381693LND
CSB-EP381693LND
CSB-BP381693LND
CSB-MP381693LND
CSB-EP381693LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Ribonuclease P protein component (rnpA) CSB-YP381694LND
CSB-EP381694LND
CSB-BP381694LND
CSB-MP381694LND
CSB-EP381694LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Putative Holliday junction resolvase (llmg_0151) CSB-YP381695LND
CSB-EP381695LND
CSB-BP381695LND
CSB-MP381695LND
CSB-EP381695LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP381696LND
CSB-EP381696LND
CSB-BP381696LND
CSB-MP381696LND
CSB-EP381696LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Fructose-1,6-bisphosphatase class 3 (fbp), partial CSB-YP381697LND
CSB-EP381697LND
CSB-BP381697LND
CSB-MP381697LND
CSB-EP381697LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Energy-coupling factor transporter ATP-binding protein EcfA2 (ecfA2) CSB-YP381698LND
CSB-EP381698LND
CSB-BP381698LND
CSB-MP381698LND
CSB-EP381698LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris N-acetyldiaminopimelate deacetylase (llmg_0292) CSB-YP381699LND
CSB-EP381699LND
CSB-BP381699LND
CSB-MP381699LND
CSB-EP381699LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP381700LND
CSB-EP381700LND
CSB-BP381700LND
CSB-MP381700LND
CSB-EP381700LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP381701LND
CSB-EP381701LND
CSB-BP381701LND
CSB-MP381701LND
CSB-EP381701LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Peptide chain release factor 3 (prfC), partial CSB-YP381702LND
CSB-EP381702LND
CSB-BP381702LND
CSB-MP381702LND
CSB-EP381702LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Lysine--tRNA ligase (lysS) CSB-YP381703LND
CSB-EP381703LND
CSB-BP381703LND
CSB-MP381703LND
CSB-EP381703LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Tyrosine--tRNA ligase (tyrS) CSB-YP381704LND
CSB-EP381704LND
CSB-BP381704LND
CSB-MP381704LND
CSB-EP381704LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris DNA ligase (ligA), partial CSB-YP381705LND
CSB-EP381705LND
CSB-BP381705LND
CSB-MP381705LND
CSB-EP381705LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Holliday junction resolvase recU (recU) CSB-YP381706LND
CSB-EP381706LND
CSB-BP381706LND
CSB-MP381706LND
CSB-EP381706LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris UPF0223 protein llmg_0515 (llmg_0515) CSB-YP381707LND
CSB-EP381707LND
CSB-BP381707LND
CSB-MP381707LND
CSB-EP381707LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris HPr kinase/phosphorylase (hprK) CSB-YP381708LND
CSB-EP381708LND
CSB-BP381708LND
CSB-MP381708LND
CSB-EP381708LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Probable potassium transport system protein kup 2 (kup2), partial CSB-YP381709LND
CSB-EP381709LND
CSB-BP381709LND
CSB-MP381709LND
CSB-EP381709LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 50S ribosomal protein L33 2 (rpmG2) CSB-YP381710LND
CSB-EP381710LND
CSB-BP381710LND
CSB-MP381710LND
CSB-EP381710LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Putative membrane protein insertion efficiency factor (llmg_0750) CSB-YP381711LND
CSB-EP381711LND
CSB-BP381711LND
CSB-MP381711LND
CSB-EP381711LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 3-dehydroquinate dehydratase (aroD) CSB-YP381712LND
CSB-EP381712LND
CSB-BP381712LND
CSB-MP381712LND
CSB-EP381712LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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