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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M5 Glutamate--tRNA ligase (gltX) CSB-YP382195SUQ
CSB-EP382195SUQ
CSB-BP382195SUQ
CSB-MP382195SUQ
CSB-EP382195SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 30S ribosomal protein S12 (rpsL) CSB-YP382196SUQ
CSB-EP382196SUQ
CSB-BP382196SUQ
CSB-MP382196SUQ
CSB-EP382196SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Undecaprenyl-diphosphatase (uppP), partial CSB-YP382197SUQ
CSB-EP382197SUQ
CSB-BP382197SUQ
CSB-MP382197SUQ
CSB-EP382197SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Trigger factor (tig) CSB-YP382198SUQ
CSB-EP382198SUQ
CSB-BP382198SUQ
CSB-MP382198SUQ
CSB-EP382198SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP382199SUQ
CSB-EP382199SUQ
CSB-BP382199SUQ
CSB-MP382199SUQ
CSB-EP382199SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Deoxyribose-phosphate aldolase (deoC) CSB-YP382200SUQ
CSB-EP382200SUQ
CSB-BP382200SUQ
CSB-MP382200SUQ
CSB-EP382200SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UPF0133 protein SpyM50270 (SpyM50270) CSB-YP382201SUQ
CSB-EP382201SUQ
CSB-BP382201SUQ
CSB-MP382201SUQ
CSB-EP382201SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 30S ribosomal protein S6 (rpsF) CSB-YP382202SUQ
CSB-EP382202SUQ
CSB-BP382202SUQ
CSB-MP382202SUQ
CSB-EP382202SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP382203SUQ
CSB-EP382203SUQ
CSB-BP382203SUQ
CSB-MP382203SUQ
CSB-EP382203SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP382204SUQ
CSB-EP382204SUQ
CSB-BP382204SUQ
CSB-MP382204SUQ
CSB-EP382204SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP382205SUQ
CSB-EP382205SUQ
CSB-BP382205SUQ
CSB-MP382205SUQ
CSB-EP382205SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 tRNA (guanine-N (7)-)-methyltransferase CSB-YP382206SUQ
CSB-EP382206SUQ
CSB-BP382206SUQ
CSB-MP382206SUQ
CSB-EP382206SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP382207SUQ
CSB-EP382207SUQ
CSB-BP382207SUQ
CSB-MP382207SUQ
CSB-EP382207SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UPF0291 protein SpyM50407 (SpyM50407) CSB-YP382208SUQ
CSB-EP382208SUQ
CSB-BP382208SUQ
CSB-MP382208SUQ
CSB-EP382208SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Glycerol kinase (glpK), partial CSB-YP382209SUQ
CSB-EP382209SUQ
CSB-BP382209SUQ
CSB-MP382209SUQ
CSB-EP382209SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP382210SUQ
CSB-EP382210SUQ
CSB-BP382210SUQ
CSB-MP382210SUQ
CSB-EP382210SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 NH (3)-dependent NAD (+) synthetase CSB-YP382211SUQ
CSB-EP382211SUQ
CSB-BP382211SUQ
CSB-MP382211SUQ
CSB-EP382211SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Cell cycle protein GpsB (gpsB) CSB-YP382212SUQ
CSB-EP382212SUQ
CSB-BP382212SUQ
CSB-MP382212SUQ
CSB-EP382212SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP382213SUQ
CSB-EP382213SUQ
CSB-BP382213SUQ
CSB-MP382213SUQ
CSB-EP382213SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UPF0374 protein SpyM50469 (SpyM50469) CSB-YP382214SUQ
CSB-EP382214SUQ
CSB-BP382214SUQ
CSB-MP382214SUQ
CSB-EP382214SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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