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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aspergillus niger Autophagy-related protein 18 (atg18) CSB-YP382175AVE
CSB-EP382175AVE
CSB-BP382175AVE
CSB-MP382175AVE
CSB-EP382175AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Putative galacturan 1,4-alpha-galacturonidase C (rgxC) CSB-YP382176AVE
CSB-EP382176AVE
CSB-BP382176AVE
CSB-MP382176AVE
CSB-EP382176AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Protein sds23 (sds23), partial CSB-YP382177AVE
CSB-EP382177AVE
CSB-BP382177AVE
CSB-MP382177AVE
CSB-EP382177AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable carboxypeptidase An18g06210 (An18g06210) CSB-YP382178AVE
CSB-EP382178AVE
CSB-BP382178AVE
CSB-MP382178AVE
CSB-EP382178AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Peptidyl-tRNA hydrolase (pth) CSB-YP382179SUQ
CSB-EP382179SUQ
CSB-BP382179SUQ
CSB-MP382179SUQ
CSB-EP382179SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP382180SUQ
CSB-EP382180SUQ
CSB-BP382180SUQ
CSB-MP382180SUQ
CSB-EP382180SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L23 (rplW) CSB-YP382181SUQ
CSB-EP382181SUQ
CSB-BP382181SUQ
CSB-MP382181SUQ
CSB-EP382181SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L22 (rplV) CSB-YP382182SUQ
CSB-EP382182SUQ
CSB-BP382182SUQ
CSB-MP382182SUQ
CSB-EP382182SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 30S ribosomal protein S3 (rpsC) CSB-YP382183SUQ
CSB-EP382183SUQ
CSB-BP382183SUQ
CSB-MP382183SUQ
CSB-EP382183SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 30S ribosomal protein S17 (rpsQ) CSB-YP382184SUQ
CSB-EP382184SUQ
CSB-BP382184SUQ
CSB-MP382184SUQ
CSB-EP382184SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 30S ribosomal protein S14 type Z (rpsZ) CSB-YP382185SUQ
CSB-EP382185SUQ
CSB-BP382185SUQ
CSB-MP382185SUQ
CSB-EP382185SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L6 (rplF) CSB-YP382186SUQ
CSB-EP382186SUQ
CSB-BP382186SUQ
CSB-MP382186SUQ
CSB-EP382186SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 30S ribosomal protein S5 (rpsE) CSB-YP382187SUQ
CSB-EP382187SUQ
CSB-BP382187SUQ
CSB-MP382187SUQ
CSB-EP382187SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L15 (rplO) CSB-YP382188SUQ
CSB-EP382188SUQ
CSB-BP382188SUQ
CSB-MP382188SUQ
CSB-EP382188SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L36 (rpmJ) CSB-YP382189SUQ
CSB-EP382189SUQ
CSB-BP382189SUQ
CSB-MP382189SUQ
CSB-EP382189SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP382190SUQ
CSB-EP382190SUQ
CSB-BP382190SUQ
CSB-MP382190SUQ
CSB-EP382190SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP382191SUQ
CSB-EP382191SUQ
CSB-BP382191SUQ
CSB-MP382191SUQ
CSB-EP382191SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 33 kDa chaperonin (hslO) CSB-YP382192SUQ
CSB-EP382192SUQ
CSB-BP382192SUQ
CSB-MP382192SUQ
CSB-EP382192SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 V-type ATP synthase beta chain (atpB) CSB-YP382193SUQ
CSB-EP382193SUQ
CSB-BP382193SUQ
CSB-MP382193SUQ
CSB-EP382193SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Leucine--tRNA ligase (leuS), partial CSB-YP382194SUQ
CSB-EP382194SUQ
CSB-BP382194SUQ
CSB-MP382194SUQ
CSB-EP382194SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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