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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactococcus lactis subsp. cremoris Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP382295LND
CSB-EP382295LND
CSB-BP382295LND
CSB-MP382295LND
CSB-EP382295LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Enolase (eno) CSB-YP382296LND
CSB-EP382296LND
CSB-BP382296LND
CSB-MP382296LND
CSB-EP382296LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Serine--tRNA ligase (serS) CSB-YP382297LND
CSB-EP382297LND
CSB-BP382297LND
CSB-MP382297LND
CSB-EP382297LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Uridine kinase (udk) CSB-YP382298LND
CSB-EP382298LND
CSB-BP382298LND
CSB-MP382298LND
CSB-EP382298LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Ferredoxin--NADP reductase (llmg_0776) CSB-YP382299LND
CSB-EP382299LND
CSB-BP382299LND
CSB-MP382299LND
CSB-EP382299LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Mannonate dehydratase (uxuA) CSB-YP382300LND
CSB-EP382300LND
CSB-BP382300LND
CSB-MP382300LND
CSB-EP382300LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Gamma-glutamyl phosphate reductase (proA) CSB-YP382301LND
CSB-EP382301LND
CSB-BP382301LND
CSB-MP382301LND
CSB-EP382301LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP382302LND
CSB-EP382302LND
CSB-BP382302LND
CSB-MP382302LND
CSB-EP382302LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris GTPase obg (obg) CSB-YP382303LND
CSB-EP382303LND
CSB-BP382303LND
CSB-MP382303LND
CSB-EP382303LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Glucosamine-6-phosphate deaminase (nagB) CSB-YP382304LND
CSB-EP382304LND
CSB-BP382304LND
CSB-MP382304LND
CSB-EP382304LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris CCA-adding enzyme (cca) CSB-YP382305LND
CSB-EP382305LND
CSB-BP382305LND
CSB-MP382305LND
CSB-EP382305LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP382306LND
CSB-EP382306LND
CSB-BP382306LND
CSB-MP382306LND
CSB-EP382306LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Tryptophan synthase beta chain (trpB) CSB-YP382307LND
CSB-EP382307LND
CSB-BP382307LND
CSB-MP382307LND
CSB-EP382307LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris UPF0122 protein llmg_1069 (llmg_1069) CSB-YP382308LND
CSB-EP382308LND
CSB-BP382308LND
CSB-MP382308LND
CSB-EP382308LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP382309LND
CSB-EP382309LND
CSB-BP382309LND
CSB-MP382309LND
CSB-EP382309LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Segregation and condensation protein A (scpA) CSB-YP382310LND
CSB-EP382310LND
CSB-BP382310LND
CSB-MP382310LND
CSB-EP382310LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 50S ribosomal protein L10 (rplJ) CSB-YP382311LND
CSB-EP382311LND
CSB-BP382311LND
CSB-MP382311LND
CSB-EP382311LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP382312LND
CSB-EP382312LND
CSB-BP382312LND
CSB-MP382312LND
CSB-EP382312LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Dihydroxy-acid dehydratase (ilvD), partial CSB-YP382313LND
CSB-EP382313LND
CSB-BP382313LND
CSB-MP382313LND
CSB-EP382313LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP382314LND
CSB-EP382314LND
CSB-BP382314LND
CSB-MP382314LND
CSB-EP382314LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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