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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Glutamate--tRNA ligase (gltX) CSB-YP382495PZG
CSB-EP382495PZG
CSB-BP382495PZG
CSB-MP382495PZG
CSB-EP382495PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Uridylate kinase (pyrH) CSB-YP382496PZG
CSB-EP382496PZG
CSB-BP382496PZG
CSB-MP382496PZG
CSB-EP382496PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (chlL) CSB-YP382497PZG
CSB-EP382497PZG
CSB-BP382497PZG
CSB-MP382497PZG
CSB-EP382497PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Threonine--tRNA ligase (thrS), partial CSB-YP382498PZG
CSB-EP382498PZG
CSB-BP382498PZG
CSB-MP382498PZG
CSB-EP382498PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 33 kDa chaperonin (hslO) CSB-YP382499PZG
CSB-EP382499PZG
CSB-BP382499PZG
CSB-MP382499PZG
CSB-EP382499PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP382500PZG
CSB-EP382500PZG
CSB-BP382500PZG
CSB-MP382500PZG
CSB-EP382500PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP382501PZG
CSB-EP382501PZG
CSB-BP382501PZG
CSB-MP382501PZG
CSB-EP382501PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phycocyanobilin:ferredoxin oxidoreductase (pcyA) CSB-YP382502PZG
CSB-EP382502PZG
CSB-BP382502PZG
CSB-MP382502PZG
CSB-EP382502PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (acsF) CSB-YP382503PZG
CSB-EP382503PZG
CSB-BP382503PZG
CSB-MP382503PZG
CSB-EP382503PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Leucine--tRNA ligase (leuS), partial CSB-YP382504PZG
CSB-EP382504PZG
CSB-BP382504PZG
CSB-MP382504PZG
CSB-EP382504PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP382505PZG
CSB-EP382505PZG
CSB-BP382505PZG
CSB-MP382505PZG
CSB-EP382505PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S15 (rpsO) CSB-YP382506PZG
CSB-EP382506PZG
CSB-BP382506PZG
CSB-MP382506PZG
CSB-EP382506PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Undecaprenyl-diphosphatase (uppP), partial CSB-YP382507PZG
CSB-EP382507PZG
CSB-BP382507PZG
CSB-MP382507PZG
CSB-EP382507PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP382508PZG
CSB-EP382508PZG
CSB-BP382508PZG
CSB-MP382508PZG
CSB-EP382508PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP382509PZG
CSB-EP382509PZG
CSB-BP382509PZG
CSB-MP382509PZG
CSB-EP382509PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP382510PZG
CSB-EP382510PZG
CSB-BP382510PZG
CSB-MP382510PZG
CSB-EP382510PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cysteine--tRNA ligase (cysS) CSB-YP382511PZG
CSB-EP382511PZG
CSB-BP382511PZG
CSB-MP382511PZG
CSB-EP382511PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 7 (petM), partial CSB-YP382512PZG
CSB-EP382512PZG
CSB-BP382512PZG
CSB-MP382512PZG
CSB-EP382512PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L34 (rpmH) CSB-YP382513PZG
CSB-EP382513PZG
CSB-BP382513PZG
CSB-MP382513PZG
CSB-EP382513PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S16 (rpsP) CSB-YP382514PZG
CSB-EP382514PZG
CSB-BP382514PZG
CSB-MP382514PZG
CSB-EP382514PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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