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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. indica 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1), partial CSB-YP383697OFF1
CSB-EP383697OFF1
CSB-BP383697OFF1
CSB-MP383697OFF1
CSB-EP383697OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Non-specific lipid-transfer protein 2 (LTP-2) CSB-YP383698OFF
CSB-EP383698OFF
CSB-BP383698OFF
CSB-MP383698OFF
CSB-EP383698OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica UPF0496 protein 3 (OsI_009784), partial CSB-YP383699OFF1
CSB-EP383699OFF1
CSB-BP383699OFF1
CSB-MP383699OFF1
CSB-EP383699OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Phytoene dehydrogenase, chloroplastic/chromoplastic (PDS1) CSB-YP383700OFF
CSB-EP383700OFF
CSB-BP383700OFF
CSB-MP383700OFF
CSB-EP383700OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX19 (HOX19) CSB-YP383701OFF
CSB-EP383701OFF
CSB-BP383701OFF
CSB-MP383701OFF
CSB-EP383701OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica L-ascorbate peroxidase 1, cytosolic (APX1) CSB-YP383702OFF
CSB-EP383702OFF
CSB-BP383702OFF
CSB-MP383702OFF
CSB-EP383702OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Pyruvate decarboxylase isozyme 2 (PDC2), partial CSB-YP383703OFF
CSB-EP383703OFF
CSB-BP383703OFF
CSB-MP383703OFF
CSB-EP383703OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Nicotianamine synthase 2 (NAS2) CSB-YP383704OFF
CSB-EP383704OFF
CSB-BP383704OFF
CSB-MP383704OFF
CSB-EP383704OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Superoxide dismutase [Cu-Zn] 1 (SODCC1) CSB-YP383705OFF
CSB-EP383705OFF
CSB-BP383705OFF
CSB-MP383705OFF
CSB-EP383705OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica COBRA-like protein 5 (BC1) CSB-YP383706OFF
CSB-EP383706OFF
CSB-BP383706OFF
CSB-MP383706OFF
CSB-EP383706OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein SHORT-ROOT 2 (SHR2), partial CSB-YP383707OFF
CSB-EP383707OFF
CSB-BP383707OFF
CSB-MP383707OFF
CSB-EP383707OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Costars family protein (OsI_012692) CSB-YP383708OFF
CSB-EP383708OFF
CSB-BP383708OFF
CSB-MP383708OFF
CSB-EP383708OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Phytochrome A (PHYA), partial CSB-YP383709OFF
CSB-EP383709OFF
CSB-BP383709OFF
CSB-MP383709OFF
CSB-EP383709OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 1-aminocyclopropane-1-carboxylate synthase 1 (ACC1) CSB-YP383710OFF
CSB-EP383710OFF
CSB-BP383710OFF
CSB-MP383710OFF
CSB-EP383710OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Phytochrome C (PHYC), partial CSB-YP383711OFF
CSB-EP383711OFF
CSB-BP383711OFF
CSB-MP383711OFF
CSB-EP383711OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Serine/threonine-protein phosphatase PP2A-2 catalytic subunit (PP2A2) CSB-YP383712OFF
CSB-EP383712OFF
CSB-BP383712OFF
CSB-MP383712OFF
CSB-EP383712OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Actin-3 (ACT3) CSB-YP383713OFF
CSB-EP383713OFF
CSB-BP383713OFF
CSB-MP383713OFF
CSB-EP383713OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Electron transfer flavoprotein subunit beta, mitochondrial (ETFB), partial CSB-YP383714OFF
CSB-EP383714OFF
CSB-BP383714OFF
CSB-MP383714OFF
CSB-EP383714OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Casparian strip membrane protein OsI_16169 (H0219H12.8, OsI_16169, OSIGBa0097P08.13), partial CSB-YP383715OFF1
CSB-EP383715OFF1
CSB-BP383715OFF1
CSB-MP383715OFF1
CSB-EP383715OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Magnesium transporter MRS2-C (H0311C03.3, OsI_16518), partial CSB-YP383716OFF1
CSB-EP383716OFF1
CSB-BP383716OFF1
CSB-MP383716OFF1
CSB-EP383716OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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