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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactococcus lactis subsp. cremoris N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP382896LND
CSB-EP382896LND
CSB-BP382896LND
CSB-MP382896LND
CSB-EP382896LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Elongation factor 4 (lepA), partial CSB-YP382897LND
CSB-EP382897LND
CSB-BP382897LND
CSB-MP382897LND
CSB-EP382897LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 50S ribosomal protein L21 (rplU) CSB-YP382898LND
CSB-EP382898LND
CSB-BP382898LND
CSB-MP382898LND
CSB-EP382898LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP382899LND
CSB-EP382899LND
CSB-BP382899LND
CSB-MP382899LND
CSB-EP382899LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris UPF0374 protein llmg_1550 (llmg_1550) CSB-YP382900LND
CSB-EP382900LND
CSB-BP382900LND
CSB-MP382900LND
CSB-EP382900LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Formate--tetrahydrofolate ligase (fhs), partial CSB-YP382901LND
CSB-EP382901LND
CSB-BP382901LND
CSB-MP382901LND
CSB-EP382901LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP382902LND
CSB-EP382902LND
CSB-BP382902LND
CSB-MP382902LND
CSB-EP382902LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP382903LND
CSB-EP382903LND
CSB-BP382903LND
CSB-MP382903LND
CSB-EP382903LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Leucine--tRNA ligase (leuS), partial CSB-YP382904LND
CSB-EP382904LND
CSB-BP382904LND
CSB-MP382904LND
CSB-EP382904LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Cystathionine beta-lyase (metC) CSB-YP382905LND
CSB-EP382905LND
CSB-BP382905LND
CSB-MP382905LND
CSB-EP382905LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Transcriptional repressor NrdR (nrdR) CSB-YP382906LND
CSB-EP382906LND
CSB-BP382906LND
CSB-MP382906LND
CSB-EP382906LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Di-/tripeptide transporter (dtpT), partial CSB-YP382907LND
CSB-EP382907LND
CSB-BP382907LND
CSB-MP382907LND
CSB-EP382907LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus quercinus Alpha-conotoxin-like Qc1.6 CSB-YP382908CXE
CSB-EP382908CXE
CSB-BP382908CXE
CSB-MP382908CXE
CSB-EP382908CXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein IN2-1 homolog B (GSTZ5) CSB-YP382909OFF
CSB-EP382909OFF
CSB-BP382909OFF
CSB-MP382909OFF
CSB-EP382909OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Septin and tuftelin-interacting protein 1 homolog (stip-1), partial CSB-YP382910DKK
CSB-EP382910DKK
CSB-BP382910DKK
CSB-MP382910DKK
CSB-EP382910DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nuphar advena ATP synthase subunit alpha, chloroplastic (atpA), partial CSB-YP382911NFE
CSB-EP382911NFE
CSB-BP382911NFE
CSB-MP382911NFE
CSB-EP382911NFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nuphar advena Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP382912NFE1
CSB-EP382912NFE1
CSB-BP382912NFE1
CSB-MP382912NFE1
CSB-EP382912NFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nuphar advena NAD (P)H-quinone oxidoreductase subunit J, chloroplastic CSB-YP382913NFE
CSB-EP382913NFE
CSB-BP382913NFE
CSB-MP382913NFE
CSB-EP382913NFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nuphar advena Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD) CSB-YP382914NFE
CSB-EP382914NFE
CSB-BP382914NFE
CSB-MP382914NFE
CSB-EP382914NFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nuphar advena Translation initiation factor IF-1, chloroplastic (infA) CSB-YP382915NFE
CSB-EP382915NFE
CSB-BP382915NFE
CSB-MP382915NFE
CSB-EP382915NFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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