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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pyogenes serotype M5 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP382796SUQ
CSB-EP382796SUQ
CSB-BP382796SUQ
CSB-MP382796SUQ
CSB-EP382796SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Uridine kinase (udk) CSB-YP382797SUQ
CSB-EP382797SUQ
CSB-BP382797SUQ
CSB-MP382797SUQ
CSB-EP382797SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Shikimate kinase (aroK) CSB-YP382798SUQ
CSB-EP382798SUQ
CSB-BP382798SUQ
CSB-MP382798SUQ
CSB-EP382798SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UvrABC system protein B (uvrB), partial CSB-YP382799SUQ
CSB-EP382799SUQ
CSB-BP382799SUQ
CSB-MP382799SUQ
CSB-EP382799SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UPF0223 protein SpyM50840 (SpyM50840) CSB-YP382800SUQ
CSB-EP382800SUQ
CSB-BP382800SUQ
CSB-MP382800SUQ
CSB-EP382800SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UPF0122 protein SpyM50882 (SpyM50882) CSB-YP382801SUQ
CSB-EP382801SUQ
CSB-BP382801SUQ
CSB-MP382801SUQ
CSB-EP382801SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Probable apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (citX) CSB-YP382802SUQ
CSB-EP382802SUQ
CSB-BP382802SUQ
CSB-MP382802SUQ
CSB-EP382802SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP382803SUQ
CSB-EP382803SUQ
CSB-BP382803SUQ
CSB-MP382803SUQ
CSB-EP382803SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Redox-sensing transcriptional repressor rex (rex) CSB-YP382804SUQ
CSB-EP382804SUQ
CSB-BP382804SUQ
CSB-MP382804SUQ
CSB-EP382804SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 50S ribosomal protein L7/L12 (rplL) CSB-YP382805SUQ
CSB-EP382805SUQ
CSB-BP382805SUQ
CSB-MP382805SUQ
CSB-EP382805SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 tRNA modification GTPase MnmE (mnmE) CSB-YP382806SUQ
CSB-EP382806SUQ
CSB-BP382806SUQ
CSB-MP382806SUQ
CSB-EP382806SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Phosphoglucosamine mutase (glmM) CSB-YP382807SUQ
CSB-EP382807SUQ
CSB-BP382807SUQ
CSB-MP382807SUQ
CSB-EP382807SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 UPF0176 protein SpyM51090 (SpyM51090) CSB-YP382808SUQ
CSB-EP382808SUQ
CSB-BP382808SUQ
CSB-MP382808SUQ
CSB-EP382808SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP382809SUQ
CSB-EP382809SUQ
CSB-BP382809SUQ
CSB-MP382809SUQ
CSB-EP382809SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Ribose-5-phosphate isomerase A (rpiA) CSB-YP382810SUQ
CSB-EP382810SUQ
CSB-BP382810SUQ
CSB-MP382810SUQ
CSB-EP382810SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP382811SUQ
CSB-EP382811SUQ
CSB-BP382811SUQ
CSB-MP382811SUQ
CSB-EP382811SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 CCA-adding enzyme (cca) CSB-YP382812SUQ
CSB-EP382812SUQ
CSB-BP382812SUQ
CSB-MP382812SUQ
CSB-EP382812SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 30S ribosomal protein S16 (rpsP) CSB-YP382813SUQ
CSB-EP382813SUQ
CSB-BP382813SUQ
CSB-MP382813SUQ
CSB-EP382813SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Lipoprotein signal peptidase (lspA), partial CSB-YP382814SUQ1
CSB-EP382814SUQ1
CSB-BP382814SUQ1
CSB-MP382814SUQ1
CSB-EP382814SUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M5 Probable tRNA sulfurtransferase (thiI) CSB-YP382815SUQ
CSB-EP382815SUQ
CSB-BP382815SUQ
CSB-MP382815SUQ
CSB-EP382815SUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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