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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus 60 kDa chaperonin 1 (groL1), partial CSB-YP382996PZB
CSB-EP382996PZB
CSB-BP382996PZB
CSB-MP382996PZB
CSB-EP382996PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamate--tRNA ligase (gltX) CSB-YP382997PZB
CSB-EP382997PZB
CSB-BP382997PZB
CSB-MP382997PZB
CSB-EP382997PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP382998PZB
CSB-EP382998PZB
CSB-BP382998PZB
CSB-MP382998PZB
CSB-EP382998PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP382999PZB
CSB-EP382999PZB
CSB-BP382999PZB
CSB-MP382999PZB
CSB-EP382999PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP383000PZB
CSB-EP383000PZB
CSB-BP383000PZB
CSB-MP383000PZB
CSB-EP383000PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glycogen synthase (glgA) CSB-YP383001PZB
CSB-EP383001PZB
CSB-BP383001PZB
CSB-MP383001PZB
CSB-EP383001PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 33 kDa chaperonin (hslO) CSB-YP383002PZB
CSB-EP383002PZB
CSB-BP383002PZB
CSB-MP383002PZB
CSB-EP383002PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome c biogenesis protein CcsA (ccsA), partial CSB-YP383003PZB
CSB-EP383003PZB
CSB-BP383003PZB
CSB-MP383003PZB
CSB-EP383003PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP383004PZB
CSB-EP383004PZB
CSB-BP383004PZB
CSB-MP383004PZB
CSB-EP383004PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease accessory protein UreE (ureE) CSB-YP383005PZB
CSB-EP383005PZB
CSB-BP383005PZB
CSB-MP383005PZB
CSB-EP383005PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Urease subunit alpha (ureC), partial CSB-YP383006PZB
CSB-EP383006PZB
CSB-BP383006PZB
CSB-MP383006PZB
CSB-EP383006PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center Psb28 protein (psb28) CSB-YP383007PZB
CSB-EP383007PZB
CSB-BP383007PZB
CSB-MP383007PZB
CSB-EP383007PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphopantetheine adenylyltransferase (coaD) CSB-YP383008PZB
CSB-EP383008PZB
CSB-BP383008PZB
CSB-MP383008PZB
CSB-EP383008PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L32 (rpmF) CSB-YP383009PZB
CSB-EP383009PZB
CSB-BP383009PZB
CSB-MP383009PZB
CSB-EP383009PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP383010PZB
CSB-EP383010PZB
CSB-BP383010PZB
CSB-MP383010PZB
CSB-EP383010PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional protein FolD (folD) CSB-YP383011PZB
CSB-EP383011PZB
CSB-BP383011PZB
CSB-MP383011PZB
CSB-EP383011PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UPF0176 protein A9601_12001 (A9601_12001) CSB-YP383012PZB
CSB-EP383012PZB
CSB-BP383012PZB
CSB-MP383012PZB
CSB-EP383012PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 7 (petM), partial CSB-YP383013PZB
CSB-EP383013PZB
CSB-BP383013PZB
CSB-MP383013PZB
CSB-EP383013PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus PKHD-type hydroxylase A9601_13531 (A9601_13531) CSB-YP383014PZB
CSB-EP383014PZB
CSB-BP383014PZB
CSB-MP383014PZB
CSB-EP383014PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribonuclease P protein component (rnpA) CSB-YP383015PZB
CSB-EP383015PZB
CSB-BP383015PZB
CSB-MP383015PZB
CSB-EP383015PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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