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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Hyperthermus butylicus 50S ribosomal protein L1P (rpl1p) CSB-YP382976HSR
CSB-EP382976HSR
CSB-BP382976HSR
CSB-MP382976HSR
CSB-EP382976HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus RNA-binding protein AU-1 (aubA) CSB-YP382977HSR
CSB-EP382977HSR
CSB-BP382977HSR
CSB-MP382977HSR
CSB-EP382977HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus 30S ribosomal protein S27e (rps27e) CSB-YP382978HSR
CSB-EP382978HSR
CSB-BP382978HSR
CSB-MP382978HSR
CSB-EP382978HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperthermus butylicus Signal recognition 54 kDa protein (srp54) CSB-YP382979HSR
CSB-EP382979HSR
CSB-BP382979HSR
CSB-MP382979HSR
CSB-EP382979HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center X protein (psbX), partial CSB-YP382980PZB1
CSB-EP382980PZB1
CSB-BP382980PZB1
CSB-MP382980PZB1
CSB-EP382980PZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit O CSB-YP382981PZB
CSB-EP382981PZB
CSB-BP382981PZB
CSB-MP382981PZB
CSB-EP382981PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit M CSB-YP382982PZB
CSB-EP382982PZB
CSB-BP382982PZB
CSB-MP382982PZB
CSB-EP382982PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit H CSB-YP382983PZB
CSB-EP382983PZB
CSB-BP382983PZB
CSB-MP382983PZB
CSB-EP382983PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA modification GTPase MnmE (mnmE) CSB-YP382984PZB
CSB-EP382984PZB
CSB-BP382984PZB
CSB-MP382984PZB
CSB-EP382984PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L7/L12 (rplL) CSB-YP382985PZB
CSB-EP382985PZB
CSB-BP382985PZB
CSB-MP382985PZB
CSB-EP382985PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L11 (rplK) CSB-YP382986PZB
CSB-EP382986PZB
CSB-BP382986PZB
CSB-MP382986PZB
CSB-EP382986PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem Q (B) protein, partial CSB-YP382987PZB1
CSB-EP382987PZB1
CSB-BP382987PZB1
CSB-MP382987PZB1
CSB-EP382987PZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Isoleucine--tRNA ligase (ileS), partial CSB-YP382988PZB
CSB-EP382988PZB
CSB-BP382988PZB
CSB-MP382988PZB
CSB-EP382988PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphoglucosamine mutase (glmM) CSB-YP382989PZB
CSB-EP382989PZB
CSB-BP382989PZB
CSB-MP382989PZB
CSB-EP382989PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit J CSB-YP382990PZB
CSB-EP382990PZB
CSB-BP382990PZB
CSB-MP382990PZB
CSB-EP382990PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein L (psbL), partial CSB-YP382991PZB
CSB-EP382991PZB
CSB-BP382991PZB
CSB-MP382991PZB
CSB-EP382991PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein M (psbM), partial CSB-YP382992PZB
CSB-EP382992PZB
CSB-BP382992PZB
CSB-MP382992PZB
CSB-EP382992PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I reaction center subunit IV (psaE) CSB-YP382993PZB
CSB-EP382993PZB
CSB-BP382993PZB
CSB-MP382993PZB
CSB-EP382993PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 3-dehydroquinate dehydratase (aroQ) CSB-YP382994PZB
CSB-EP382994PZB
CSB-BP382994PZB
CSB-MP382994PZB
CSB-EP382994PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable malate:quinone oxidoreductase (mqo) CSB-YP382995PZB
CSB-EP382995PZB
CSB-BP382995PZB
CSB-MP382995PZB
CSB-EP382995PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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