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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylibium petroleiphilum 50S ribosomal protein L17 (rplQ) CSB-YP384777MNX
CSB-EP384777MNX
CSB-BP384777MNX
CSB-MP384777MNX
CSB-EP384777MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S11 (rpsK) CSB-YP384778MNX
CSB-EP384778MNX
CSB-BP384778MNX
CSB-MP384778MNX
CSB-EP384778MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Translation initiation factor IF-1 (infA) CSB-YP384779MNX
CSB-EP384779MNX
CSB-BP384779MNX
CSB-MP384779MNX
CSB-EP384779MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S8 (rpsH) CSB-YP384780MNX
CSB-EP384780MNX
CSB-BP384780MNX
CSB-MP384780MNX
CSB-EP384780MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S3 (rpsC) CSB-YP384781MNX
CSB-EP384781MNX
CSB-BP384781MNX
CSB-MP384781MNX
CSB-EP384781MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 30S ribosomal protein S10 (rpsJ) CSB-YP384782MNX
CSB-EP384782MNX
CSB-BP384782MNX
CSB-MP384782MNX
CSB-EP384782MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP384783MNX
CSB-EP384783MNX
CSB-BP384783MNX
CSB-MP384783MNX
CSB-EP384783MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Anthranilate phosphoribosyltransferase (trpD) CSB-YP384784MNX
CSB-EP384784MNX
CSB-BP384784MNX
CSB-MP384784MNX
CSB-EP384784MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Glycerol kinase (glpK) CSB-YP384785MNX
CSB-EP384785MNX
CSB-BP384785MNX
CSB-MP384785MNX
CSB-EP384785MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP384786MNX1
CSB-EP384786MNX1
CSB-BP384786MNX1
CSB-MP384786MNX1
CSB-EP384786MNX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP384787MNX
CSB-EP384787MNX
CSB-BP384787MNX
CSB-MP384787MNX
CSB-EP384787MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Ribonuclease HII 1 (rnhB1) CSB-YP384788MNX
CSB-EP384788MNX
CSB-BP384788MNX
CSB-MP384788MNX
CSB-EP384788MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP384789MNT
CSB-EP384789MNT
CSB-BP384789MNT
CSB-MP384789MNT
CSB-EP384789MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum 30S ribosomal protein S19P (rps19p) CSB-YP384790MNT
CSB-EP384790MNT
CSB-BP384790MNT
CSB-MP384790MNT
CSB-EP384790MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum 30S ribosomal protein S8P (rps8p) CSB-YP384791MNT
CSB-EP384791MNT
CSB-BP384791MNT
CSB-MP384791MNT
CSB-EP384791MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum 2-phospho-L-lactate transferase (cofD) CSB-YP384792MNT
CSB-EP384792MNT
CSB-BP384792MNT
CSB-MP384792MNT
CSB-EP384792MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum Isoleucine--tRNA ligase (ileS), partial CSB-YP384793MNT
CSB-EP384793MNT
CSB-BP384793MNT
CSB-MP384793MNT
CSB-EP384793MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum 5'-nucleotidase surE (surE) CSB-YP384794MNT
CSB-EP384794MNT
CSB-BP384794MNT
CSB-MP384794MNT
CSB-EP384794MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum 30S ribosomal protein S3Ae (rps3Ae) CSB-YP384795MNT
CSB-EP384795MNT
CSB-BP384795MNT
CSB-MP384795MNT
CSB-EP384795MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum 50S ribosomal protein L10e (rpl10e) CSB-YP384796MNT
CSB-EP384796MNT
CSB-BP384796MNT
CSB-MP384796MNT
CSB-EP384796MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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