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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. indica Probable pyruvate, phosphate dikinase regulatory protein, chloroplastic (PDRP1) CSB-YP383738OFF
CSB-EP383738OFF
CSB-BP383738OFF
CSB-MP383738OFF
CSB-EP383738OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Ribosome-recycling factor, chloroplastic (OsI_26546) CSB-YP383739OFF
CSB-EP383739OFF
CSB-BP383739OFF
CSB-MP383739OFF
CSB-EP383739OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable 6-phosphogluconolactonase 2 (OsI_025867) CSB-YP383740OFF
CSB-EP383740OFF
CSB-BP383740OFF
CSB-MP383740OFF
CSB-EP383740OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable histone-arginine methyltransferase CARM1 (CARM1), partial CSB-YP383741OFF
CSB-EP383741OFF
CSB-BP383741OFF
CSB-MP383741OFF
CSB-EP383741OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Pyruvate decarboxylase isozyme 3 (PDC3), partial CSB-YP383742OFF
CSB-EP383742OFF
CSB-BP383742OFF
CSB-MP383742OFF
CSB-EP383742OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica CASP-like protein OsI_27482 (OsI_27482), partial CSB-YP383743OFF1
CSB-EP383743OFF1
CSB-BP383743OFF1
CSB-MP383743OFF1
CSB-EP383743OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Actin-related protein 4 (ARP4) CSB-YP383744OFF
CSB-EP383744OFF
CSB-BP383744OFF
CSB-MP383744OFF
CSB-EP383744OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Acyl-[acyl-carrier-protein] desaturase 7, chloroplastic (OsI_28174) CSB-YP383745OFF
CSB-EP383745OFF
CSB-BP383745OFF
CSB-MP383745OFF
CSB-EP383745OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica DEAD-box ATP-dependent RNA helicase 29 (OsI_028228), partial CSB-YP383746OFF
CSB-EP383746OFF
CSB-BP383746OFF
CSB-MP383746OFF
CSB-EP383746OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX27 (HOX27) CSB-YP383747OFF
CSB-EP383747OFF
CSB-BP383747OFF
CSB-MP383747OFF
CSB-EP383747OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica CASP-like protein OsI_30044 (OsI_30044), partial CSB-YP383748OFF1
CSB-EP383748OFF1
CSB-BP383748OFF1
CSB-MP383748OFF1
CSB-EP383748OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Retinoblastoma-related protein 1 (RBR1), partial CSB-YP383749OFF
CSB-EP383749OFF
CSB-BP383749OFF
CSB-MP383749OFF
CSB-EP383749OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Bidirectional sugar transporter SWEET7b (SWEET7B), partial CSB-YP383750OFF1
CSB-EP383750OFF1
CSB-BP383750OFF1
CSB-MP383750OFF1
CSB-EP383750OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1) CSB-YP383751OFF
CSB-EP383751OFF
CSB-BP383751OFF
CSB-MP383751OFF
CSB-EP383751OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX15 (HOX15) CSB-YP383752OFF
CSB-EP383752OFF
CSB-BP383752OFF
CSB-MP383752OFF
CSB-EP383752OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica SPX domain-containing protein 3 (SPX3) CSB-YP383753OFF
CSB-EP383753OFF
CSB-BP383753OFF
CSB-MP383753OFF
CSB-EP383753OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Cyanate hydratase (CYN) CSB-YP383754OFF
CSB-EP383754OFF
CSB-BP383754OFF
CSB-MP383754OFF
CSB-EP383754OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable protein arginine N-methyltransferase 6.2 (PRMT6.2) CSB-YP383755OFF
CSB-EP383755OFF
CSB-BP383755OFF
CSB-MP383755OFF
CSB-EP383755OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Putative magnesium transporter MRS2-G (MRS2-G), partial CSB-YP383756OFF1
CSB-EP383756OFF1
CSB-BP383756OFF1
CSB-MP383756OFF1
CSB-EP383756OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Serine/threonine-protein kinase SAPK3 (SAPK3) CSB-YP383757OFF
CSB-EP383757OFF
CSB-BP383757OFF
CSB-MP383757OFF
CSB-EP383757OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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