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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acinetobacter baumannii Ribonuclease PH (rph) CSB-YP384137AUD
CSB-EP384137AUD
CSB-BP384137AUD
CSB-MP384137AUD
CSB-EP384137AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP384138AUD
CSB-EP384138AUD
CSB-BP384138AUD
CSB-MP384138AUD
CSB-EP384138AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable cytosol aminopeptidase (pepA) CSB-YP384139AUD
CSB-EP384139AUD
CSB-BP384139AUD
CSB-MP384139AUD
CSB-EP384139AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable Fe (2+)-trafficking protein CSB-YP384140AUD
CSB-EP384140AUD
CSB-BP384140AUD
CSB-MP384140AUD
CSB-EP384140AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP384141AUD
CSB-EP384141AUD
CSB-BP384141AUD
CSB-MP384141AUD
CSB-EP384141AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ribosome-binding factor A (rbfA) CSB-YP384142AUD
CSB-EP384142AUD
CSB-BP384142AUD
CSB-MP384142AUD
CSB-EP384142AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L31 type B (rpmE2) CSB-YP384143AUD
CSB-EP384143AUD
CSB-BP384143AUD
CSB-MP384143AUD
CSB-EP384143AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP384144AUD
CSB-EP384144AUD
CSB-BP384144AUD
CSB-MP384144AUD
CSB-EP384144AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L28 (rpmB) CSB-YP384145AUD
CSB-EP384145AUD
CSB-BP384145AUD
CSB-MP384145AUD
CSB-EP384145AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Sec-independent protein translocase protein TatA (tatA), partial CSB-YP384146AUD
CSB-EP384146AUD
CSB-BP384146AUD
CSB-MP384146AUD
CSB-EP384146AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Trigger factor (tig) CSB-YP384147AUD
CSB-EP384147AUD
CSB-BP384147AUD
CSB-MP384147AUD
CSB-EP384147AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP384148AUD
CSB-EP384148AUD
CSB-BP384148AUD
CSB-MP384148AUD
CSB-EP384148AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Histidine--tRNA ligase (hisS) CSB-YP384149AUD
CSB-EP384149AUD
CSB-BP384149AUD
CSB-MP384149AUD
CSB-EP384149AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Threonine--tRNA ligase (thrS), partial CSB-YP384150AUD
CSB-EP384150AUD
CSB-BP384150AUD
CSB-MP384150AUD
CSB-EP384150AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP384151AUD
CSB-EP384151AUD
CSB-BP384151AUD
CSB-MP384151AUD
CSB-EP384151AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Darcynin 1 (A1S_0741) CSB-YP384152AUD
CSB-EP384152AUD
CSB-BP384152AUD
CSB-MP384152AUD
CSB-EP384152AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP384153AUD
CSB-EP384153AUD
CSB-BP384153AUD
CSB-MP384153AUD
CSB-EP384153AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Uracil phosphoribosyltransferase (upp) CSB-YP384154AUD
CSB-EP384154AUD
CSB-BP384154AUD
CSB-MP384154AUD
CSB-EP384154AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP384155AUD
CSB-EP384155AUD
CSB-BP384155AUD
CSB-MP384155AUD
CSB-EP384155AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Glutamyl-tRNA reductase (hemA) CSB-YP384156AUD
CSB-EP384156AUD
CSB-BP384156AUD
CSB-MP384156AUD
CSB-EP384156AUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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