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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP385137STL
CSB-EP385137STL
CSB-BP385137STL
CSB-MP385137STL
CSB-EP385137STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent protease subunit HslV (hslV) CSB-YP385138STL
CSB-EP385138STL
CSB-BP385138STL
CSB-MP385138STL
CSB-EP385138STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Sec-independent protein translocase protein TatA (tatA), partial CSB-YP385139STL
CSB-EP385139STL
CSB-BP385139STL
CSB-MP385139STL
CSB-EP385139STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent RNA helicase rhlB (rhlB) CSB-YP385140STL
CSB-EP385140STL
CSB-BP385140STL
CSB-MP385140STL
CSB-EP385140STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein CyaY (cyaY) CSB-YP385141STL
CSB-EP385141STL
CSB-BP385141STL
CSB-MP385141STL
CSB-EP385141STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Phosphoheptose isomerase (gmhA) CSB-YP385142STL
CSB-EP385142STL
CSB-BP385142STL
CSB-MP385142STL
CSB-EP385142STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica CinA-like protein (Sbal_4127) CSB-YP385143STL
CSB-EP385143STL
CSB-BP385143STL
CSB-MP385143STL
CSB-EP385143STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP385144STL
CSB-EP385144STL
CSB-BP385144STL
CSB-MP385144STL
CSB-EP385144STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S13 (rpsM) CSB-YP385145STL
CSB-EP385145STL
CSB-BP385145STL
CSB-MP385145STL
CSB-EP385145STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S8 (rpsH) CSB-YP385146STL
CSB-EP385146STL
CSB-BP385146STL
CSB-MP385146STL
CSB-EP385146STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L14 (rplN) CSB-YP385147STL
CSB-EP385147STL
CSB-BP385147STL
CSB-MP385147STL
CSB-EP385147STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L22 (rplV) CSB-YP385148STL
CSB-EP385148STL
CSB-BP385148STL
CSB-MP385148STL
CSB-EP385148STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S19 (rpsS) CSB-YP385149STL
CSB-EP385149STL
CSB-BP385149STL
CSB-MP385149STL
CSB-EP385149STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L3 (rplC) CSB-YP385150STL
CSB-EP385150STL
CSB-BP385150STL
CSB-MP385150STL
CSB-EP385150STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glutamate racemase (murI) CSB-YP385151STL
CSB-EP385151STL
CSB-BP385151STL
CSB-MP385151STL
CSB-EP385151STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 33 kDa chaperonin (hslO) CSB-YP385152STL
CSB-EP385152STL
CSB-BP385152STL
CSB-MP385152STL
CSB-EP385152STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Imidazolonepropionase (hutI) CSB-YP385153STL
CSB-EP385153STL
CSB-BP385153STL
CSB-MP385153STL
CSB-EP385153STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP synthase epsilon chain (atpC) CSB-YP385154STL
CSB-EP385154STL
CSB-BP385154STL
CSB-MP385154STL
CSB-EP385154STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Putative membrane protein insertion efficiency factor (Sbal_4381) CSB-YP385155STL
CSB-EP385155STL
CSB-BP385155STL
CSB-MP385155STL
CSB-EP385155STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Biotin synthase (bioB) CSB-YP385156DUT
CSB-EP385156DUT
CSB-BP385156DUT
CSB-MP385156DUT
CSB-EP385156DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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