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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 5b Bifunctional protein pyrR (pyrR) CSB-YP385537AUF
CSB-EP385537AUF
CSB-BP385537AUF
CSB-MP385537AUF
CSB-EP385537AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b GTPase Der (der), partial CSB-YP385538AUF
CSB-EP385538AUF
CSB-BP385538AUF
CSB-MP385538AUF
CSB-EP385538AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Formate--tetrahydrofolate ligase (fhs), partial CSB-YP385539AUF
CSB-EP385539AUF
CSB-BP385539AUF
CSB-MP385539AUF
CSB-EP385539AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Tryptophan synthase beta chain (trpB) CSB-YP385540AUF
CSB-EP385540AUF
CSB-BP385540AUF
CSB-MP385540AUF
CSB-EP385540AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 30S ribosomal protein S2 (rpsB) CSB-YP385541AUF
CSB-EP385541AUF
CSB-BP385541AUF
CSB-MP385541AUF
CSB-EP385541AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP385542AUF
CSB-EP385542AUF
CSB-BP385542AUF
CSB-MP385542AUF
CSB-EP385542AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Bifunctional protein GlmU (glmU) CSB-YP385543AUF
CSB-EP385543AUF
CSB-BP385543AUF
CSB-MP385543AUF
CSB-EP385543AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 50S ribosomal protein L13 (rplM) CSB-YP385544AUF
CSB-EP385544AUF
CSB-BP385544AUF
CSB-MP385544AUF
CSB-EP385544AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UPF0246 protein APL_0602 (APL_0602) CSB-YP385545AUF
CSB-EP385545AUF
CSB-BP385545AUF
CSB-MP385545AUF
CSB-EP385545AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP385546AUF
CSB-EP385546AUF
CSB-BP385546AUF
CSB-MP385546AUF
CSB-EP385546AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribosome-binding factor A (rbfA) CSB-YP385547AUF
CSB-EP385547AUF
CSB-BP385547AUF
CSB-MP385547AUF
CSB-EP385547AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Carbon storage regulator homolog (csrA) CSB-YP385548AUF
CSB-EP385548AUF
CSB-BP385548AUF
CSB-MP385548AUF
CSB-EP385548AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Asparagine--tRNA ligase (asnS) CSB-YP385549AUF
CSB-EP385549AUF
CSB-BP385549AUF
CSB-MP385549AUF
CSB-EP385549AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP385550AUF
CSB-EP385550AUF
CSB-BP385550AUF
CSB-MP385550AUF
CSB-EP385550AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP385551AUF
CSB-EP385551AUF
CSB-BP385551AUF
CSB-MP385551AUF
CSB-EP385551AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Amino-acid acetyltransferase (argA) CSB-YP385552AUF
CSB-EP385552AUF
CSB-BP385552AUF
CSB-MP385552AUF
CSB-EP385552AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b UPF0265 protein APL_0709 (APL_0709) CSB-YP385553AUF
CSB-EP385553AUF
CSB-BP385553AUF
CSB-MP385553AUF
CSB-EP385553AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Chorismate synthase (aroC) CSB-YP385554AUF
CSB-EP385554AUF
CSB-BP385554AUF
CSB-MP385554AUF
CSB-EP385554AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP385555AUF
CSB-EP385555AUF
CSB-BP385555AUF
CSB-MP385555AUF
CSB-EP385555AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 5b Phosphoheptose isomerase (gmhA) CSB-YP385556AUF
CSB-EP385556AUF
CSB-BP385556AUF
CSB-MP385556AUF
CSB-EP385556AUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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